Preparation Of SERS Probe With Superparticle Structure And Its Sensing Application

Surface-enhanced Raman scattering(SERS)spectroscopy detection technology has the advantages of high sensitivity,strong spectral multiplexing ability,and good optical stability.Therefore,it has been widely used in the fields of disease diagnosis,environmental monitoring and food safety.The development of nano-substrates with high SERS activity and the improvement of detection sensitivity have always been one of the hot spots in the field of SERS research.Among them,the superparticle structure with a high-density hot spot area,which is synthesized by the orderly assembly of monodisperse gold nanoparticles,can generate a highintensity local electromagnetic field,so it has a good potential in highly-sensitive SERS detection.In this paper,superparticle nanostructures are used as the core to construct superparticle SERS probes for leukemia cell detection through biochemical modification.At the same time,the target cells are collected by biomodified nanomagnetic spheres to realize the diagnosis of leukemia.First,the application of SERS probe based on gold core and silver shell structure in cell detection was studied.Two types of leukemia cells were used as models.Leukemia typing and diagnosis have been achieved by combining SERS probes with a microfluic chip embedded with an ordered nano-pillar structure of silver substrate.Second,superparticle nanostructures with high SERS activity were prepared.Supernparticles with highly uniform gaps were prepared by the oil-in-water microemulsion method,using 5 nm monomer gold nanoparticles as the building unit.This structure has a higher SERS enhancement effect than gold core silver shell nanospheres.Finally,the SERS probe was constructed with the superparticle nanostructure as the core,which realized the rapidly and highly sensitive detection of leukemia cells,and was successfully applied to the analysis in clinical whole blood samples.The research results fully demonstrated the potential of the detection platform based on the superparticle structure SERS probe in the initial screening and diagnosis of leukemia,and provided a new idea for the development of a multifunctional SERS disease detection platform.