| Prilla frutescens(L.)Brittt.is a kind of multi-purpose crop which is both food and medicine.Due to its high content of polyunsaturated fatty acids,Perilla has a variety of physiological active substances,such as hypoglycemic,antioxidant,anticancer and other physiological functions.And it is known as one of the vegetable oils with the highest content of ?-linolenic acid.?-linolenic acid is a series of n-3 unsaturated fatty acids,which has the effects of reducing blood lipid,lowering blood sugar,improving memory,anti-cancer,anti-inflammatory and lowering blood pressure.However,owing to its extreme instability and susceptibility to oxidative deterioration,its embedding by microencapsulation technology can prevent the dispersion of nutrients and improve stability.In this paper,the extraction process of ?-linolenic acid from perilla oil was optimized by ultrasound-assisted ?-cyclodextrin extraction;the obtained ?-linolenic acid-rich extract(AE)was encapsulated by spray drying method,after which its physicochemical properties and activity were investigated.The results of the study were as follows.1.Using perilla oil as raw material,the process of ultrasound-assisted ?-cyclodextrin extraction of ?-linolenic acid was optimized by single-factor and response surface experiments,the best extraction process conditions were determined as follows: ultrasound temperature 51 ?,ultrasound time 68 min,liquid to material ratio 15:1,and the purity of ?-linolenic acid in the extract was 72.93% under these conditions.2.Optimized process conditions for the production of AE microcapsules on the basis of single-factor experiments with the encapsulation rate as the index.The best microcapsule process conditions were wall-to-core ratio of 5:1,solids content of 27% and homogenization time of 5.4min,and the encapsulation rate of microcapsules was 80.12% under these conditions.3.Evaluated the physicochemical indicators and properties of AE and AE microcapsules.The results showed that the prepared AE microcapsules had fine powder,low moisture content and excellent solubility;the powder was spherical,without cracks and holes;the infrared spectral detection found that the microcapsules and ?-linolenic acid contained the same characteristic spectral bands,indicating that the core and wall molecules formed a good bond;however,the zetapotential analysis showed that the microcapsules had low potential and the intermolecular aggregation was easy to occur,which might lead to the instability of the samples.The results of accelerated oxidation experiments by Schaal oven method showed that the peroxide value(POV)of AE was 8.79 times higher than that of microcapsules and the Thiobarbituric acid reactive substance(TBARS)value was 2.63 times higher than that of microcapsules after 7 d of accelerated oxidation,which indicated that microencapsulation of AE could extend shelf life.And the oxidation kinetic model of AE and microcapsules showed that both were first-order oxidation kinetic models,which had a good degree of fit.4.The hypolipidemic and anticancer activities of perilla oil,AE and microcapsules were investigated by in vitro cellular assays.The hypolipidemic assay showed that the intracellular Total Cholesterol(TC),Triglycerides(TG),Low-Density Lipoprotein Cholesterol(LDL-C),HighDensity Lipoprotein Cholesterol(HDL-C)and Reactive Oxygen Species(ROS)were significantly different between the hyperlipidemic model group and the blank control group(P<0.05).Perilla oil,AE and microcapsules all had different degrees of effect on reducing TC,TG,LDL-C and ROS content and elevating HDL-C content in high-fat Hep G2 cells;the apoptosis experiments showed that perilla oil,AE and microcapsules could induce apoptosis in Hep G2 cells to some extent after treatment,playing an anti-cancer effect. |
PDF Full Text Request