Study On Antibiotic Detection Methods Based On Lanthanide Metal-Organic Coordination Polymers

Antibiotics,as excellent antibacterial drugs,are widely used in some fields,such as treating or preventing human infectious diseases,animal husbandry and aquaculture.However,the overuse or abuse of antibiotics leads to their residues in the aquatic environment,and these residues eventually enter the food chain involved in human metabolism.The long-term ingestion of antibiotics poses a threat to human health.Therefore,the development of methods with high sensitivity,real-time and in-situ monitoring of antibiotic residues in aquatic environments should be paid attention.In recent years,fluorescent sensors based on lanthanide metal-organic coordination polymers(Ln-CPs),as a new analytical technology which has many unique advantages,have been favored by researchers.At the same time,Ln-CPs as an inorganic-organic hybrid material retain the characteristics of inorganic and organic components.It possesses the special optical properties of Ln³⁺and the controllability and porous properties of the inherent characteristics of coordination polymers.In this paper,a fluorescent sensor was designed based on Ln-CPs nanomaterials to realize real-time and in-situ monitoring of residual antibiotics in aquatic environment.First of all,a smartphone sensing platform based on Eu(TATB)fluorescent test paper was proposed for on-site detection of sulfonamide antibiotics,which provides a reliable and effective method for environment monitoring.Secondly,a pH-regulated BDC-NH₂-Tb-AMP ICPs sensor array for the rapid identification and detection of five quinolone antibiotics.This paper mainly includes the following three parts:Chapter 1.IntroductionWe first briefly introduced the general situation of antibiotics,including the classification of antibiotics,the status of use and their hazards,as well as the commonly used methods for detecting antibiotics.Then,it focuses on the concept,luminescent principle and applications in fluorescent sensors of Ln-CPs.Finally,the research significance and contents of this paper are pointed out.Chapter 2.Lanthanide metal-organic framework as a paper strip sensor for visual detection of sulfonamide with smartphone-based point-of-care platformA nanoscale lanthanide metal-organic framework Eu(TATB)with a stable red luminescence in aqueous solution is synthesized by the microemulsion method.Eu(TATB)can be used for sensitively and rapidly specific recognition of SMZ with a wide detection concentration range(0?M to 40?M)and low detection limit(0.67?M).In addition,a Point-of-Care Testing(POCT)sensing platform based on Eu(TATB)fluorescent test paper combined with smartphone imaging cassette is constructed for the visual detection of SMZ with the purpose of in-situ,real-time and accuracy.This study opens up a new avenue for the luminescent MOFs in the field of visual detection based on paper sensors as well as on-site/on-line and accurate detection of environmental pollutants.Chapter 3.pH-regulated BDC-NH2-Tb-AMP ICPs sensor array for identification and detection of quinolone antibioticsWe constructed dual-ligand lanthanide metal-organic infinite polymers(BDC-NH₂-Tb-AMP ICPs)with dual fluorescent emission centers.Quinolone antibiotics(QNs)can act as"antenna molecules"to sensitize the fluorescence of Tb³⁺,but QNs have different p Ka values,there is a difference in the sensitization effects on Tb³⁺under different pH conditions.Therefore,a pH-regulated BDC-NH₂-Tb-AMP ICPs sensor array is proposed using a simple and powerful strategy of pH regulation.The designed sensor array can not only realize pattern recognition and systematic analysis of five QNs,but also can realize the detection of antibiotics in actual water samples,confirming its reliability and practicality in practical analysis.In addition,it is expected that the fluorescence sensor based on BDC-NH₂-Tb-AMP ICPs is used to realize visual detection of QNs under UV light,which provides a new method for on-site/on-line and accurate monitoring of antibiotic residues in the environment.