Study On Innovative Imaging Analysis And Biopsy Technique Of Cancers Based On Molecular Imprinting And Surface-enhanced Raman Scattering

Global cancer fatalities account for millions of people each year and with the number of new cases expected to increase annually to 22 million by 2030,the importance of early disease identification and treatment has never been such a pertinent issue.Many that kind of cis-diol containing biomolecules have become key cancer biomarkers.The elevated expression of sialic acid containing glycoproteins is cancer progression.Such as CEA,PSA and AFP,glycoproteins have been considered the important disease markers.Therefore detecting such cis-diol molecules can realize early diagnosis and treatment of cancer.However,all existing methods for specific recognition and separation of cis-diol containing biomolecules have many deficiencies.Hydrazine chemistry,hydrophilic interaction chromatography and ion exchange chromatography method are susceptible to interference.Poor storagy stability,hard to purification and preparation,exorbitant price,low reuse efficiency,and harsh operated condition for antibody,aptamer,and lectin,although them have excellent specific recognition property.Therefore,novel alternatives that can avoid these drawbacks are highly desirable.Boron affinity molecule imprinting biomimetic materials as an alternative to antibodies have not only well preserved specificity and binding force of antibody,but also been more stable and cheaper.Because most of cis-diol containing biomolecules are low abundance molecules,selecting a fast and sensitive analysis method is very important.Surface-enhanced Raman scattering has many advantages including ultrahigh sensitivity,less susceptible to sample environment,rapid readout speed.Comparing to the fluorescence imaging,SERS imaging has a better ability to multiplex and the background signal is small.In the direct detection of the living body samples,SERS has high sensitivity.The association of these two methods has a very broad application prospects in detecting low abundance cis-diol biomolecules.First,combining boronate affinity effect,surface molecular imprinting and SERS detection,we present for the first time molecularly imprinted SERS nanotags for the imaging of cancer cells and tissues over normal cells and tissues.The introduction of molecular imprinting endowed SERS tags with high specificity toward a specific cancer biomarker,SA in this study,thus enabling the selective recognition of cancer cells and tissues.The molecular imprinted SERS nanotags overcame the disadvantages associated with the use of biomolecules particularly antibodies.As the molecularly-imprinted SERS nanotags can be extended to other cancer biomarkers or molecular signatures.Therefore,molecularly-imprinted nanotags based SERS imaging technique can be a promising tool for rapid cancer screening and cancer-related studies.Secondly,we prepared a rapid PERS(plasmon-enhanced Raman scattering)detecition of cancer biomarker proteins in living animals.The probe is similar to acupuncture needles which can directly extract specific cancer biomarker protein from the diseased tissue.And then we detected the content using sensitive optical detection means PERS,determining positive or negative sign according to the biomarker expression levels detected in tissues.In this method,the tissue without any treatment can be directly used for the extraction,immune probe can be directly used for PERS detection after a simple cleaning and objectively reflect the tissue lesions by the raman signals.Therefore,this method will have greater application in terms of diagnosis and treatment of cancer.