Synthesis Of Long-wavelength Emission Fluorescent Probes And Their Recognition Performance For H₂S,CN^- And Hcy/Cys

In this paper,four types of probes with simple structure and long-wavelength emssion are designed and synthesized based on4-diethylaminosalicylic aldehyde.The recognition performance and application of these fluorescent probes are summarized as follows:1.A novel fluorescent probe L1 with high selectivity and sensitivity to recognize H₂S in DMF/HEPES（4/6,v/v,pH=7.4）solution was synthesized.L1 can detect H₂S in actual water samples and red wine samples,and image H₂S in living cells.In addition,the L1-doped polylactic acid（PLA）nanofibrous film prepared by electrospinning can be used to detect H₂S gas.2.A high-sensitivity H₂S fluorescent probe L2 was synthesized.The detection limit of L2 is as low as 3.65×10^-7mol/L,and the reaction time of L2detecting H₂S is 5 s in DMF/PBS（4/6,v/v,pH=7.4）solution.L2 can be applied to the detection of H₂S in actual water and wine samples.In addition,the probe L2 can target mitochondria and image H₂S in mitochondria of living cells.3.A bifunctional fluorescent probe L3 was synthesized.Probe L3recognizes H₂S with fluorescence“Off-On”responding in DMF/HEPES（4/6,v/v,pH=7.4）solution.The probe can image H₂S in living cells.Moreover,L3detects CN^-by the naked eyes in DMF/PBS（1/1,v/v,pH=7.4）solution,and the color of L3 changes from purple to colorless.Probe L3 can be used to detect CN^-in germinated potatoes.4.A dual-emission fluorescent probe L4 was synthesized.L4 can recognize Hcy/Cys in THF/PBS（2/8,v/v,pH=6-9）solution with fast response,and image Hcy in living cells.In addition,L4 can individually recognize Hcy and distinguish Hcy from Cys in THF/PBS（2/8,v/v,pH 10）with high sensitivity and anti-interference ability.