Preparation And Evaluation Of Compound Pine Needles Traditional Chinese Medicine Hair Growth Solution

Seborrheic alopecia and alopecia areata are common diseases in dermatology as well as frequently-occurring and refractory diseases.Although it will not endanger people’s lives,due to the part where it occurs severely damaging the appearance of the patient,it will have a severe impact on the patient’s social and occupational functioning and bring tremendous psychological burden and mental stress to the patient.Seborrheic alopecia is a progressive and reduced hair loss disease which occurs to both men and women during adolescence and late adolescence.In China,21.3%of men and 6.0%of women suffer from the disease.ChineseTraditional Medicine has a long history of hair loss treatment,which is recorded in ancient literature.The core of Chinese Traditional Medicine treatment of hair loss is treatment based on syndrome differentiation,which means oral drug,external treatment or the combination of the two.Although Chinese Traditional Medicine being applied to the treatment of seborrheic alopecia has a long history and there are many prescriptions and folk remedies of hair loss treatment by Chinese medicinal herb,there is still a lack of effective preparations which is convenient for daily use.Hair loss is clinically classified into different types and treated differently according to different syndromes.The medicine should be prescribed by doctors and is mainly taken orally.In order to change the one-sided seborrheic alopecia treatment by modern medicine or traditional Chinese medicine,or the simple combination of the two,we started to look for a kind of traditional Chinese medicine that can conform to both the theory of traditional Chinese medicine and the pharmacology and efficacy of modern medicine.It takes pine needles and tea leaves as principal drugs to clear heat and relieve exterior syndromes,ginseng,Rhodiola rosea and glossy ganoderma as ministerial drugs to warm and nourish qi and blood,as well as to nourish liver and kidney,pepper and ginger as adjuvant drugs to promote blood circulation and remove blood stasis,as well as to dredge main and collateral channels,and cinnamon as the envoy drug to recuperate depleted yang.The formula takes procyanidins which is contained in pine needles as the detecting index,establishes the procyanidins detecting method and uses topical penetration to uptake compound pine needle hair-growth solution for convenient preparation process.The procyanidins detecting method:the chromatographic column is Baseline C18(250mm×4.6mm;5μm);mobile phase:phosphoric acid aqueous solution(pH4.5)-methanol(90:10);flow rate:0.35mL/min;detecting wavelength:200nm;column temperature:25℃;the chromatogram shows that the procyanidins are well separated with the sample size of 5.15-103μg/mL,which has a good linear relationship with the peak area within the concentration range.The equation of linear regression and correlation coefficient are Y=3532.2x+70340 and R2=0.996,the average recovery rate of solution is 98.49%and the RSD is 1.12%.In research of the therapeutic effect and safety of compound pine needle hair-growth solution treatment of mild to moderate hair loss through clinical efficacy observation,we aim to provide an effective and safe drug which is suitable for long-term use for patients with seborrheic alopecia.To judge the efficacy of the hair-growth solution,we chose patients with mild to moderate hair loss through clinical screening,asked them to take the compound traditional Chinese medicine hair-growth solution that contains 5%procyanidins 1-3mL per time,twice a day and for 3 continuous months,and then analyzed the index scores before and after use according to the patients’ scalp oil secretion degree,hair pruritus degree,dandruff degree,the degree of red pimples on the head,hair loss degree and new hair growth degree t.After 3 months of clinical observation in 688 patients that conform to the screening criteria,the marked improvement rate and the cure rate reached to 22.39%and the overall response rate was 78.99%.It is preliminarily thought that the selected traditional Chinese medicine compound can obviously promote the growth of hair follicles and extend the production period of hair follicles,so as to have a certain effect on hair loss.