Investigation On Chromatographic Fingerprint Of Traditional Chinese Medicine

In this thesis, high performance liquid chromatography (HPLC) was applied to determine the contents of active components and to establish its chromatographic fingerprint in Traditional Chinese Medicine (TCM) by electrochemical detector (ECD) and diode array detector (DAD). Those studies are very important to control the quality of TCM, and can offer a basis for the Good Agricultural Practice (GAP) management. This thesis consisted of five chapters, and the author's contributions are in the following four aspects:1. The chromatographic fingerprint of gynostemma pentaphyllum makino in different season and its different part was established by HPLC. The experimental results showed that the chromatograms of gynostemma pentaphyllum makino were obviously different in all samples, and the content is different in all samples. This investigation can provide a basis for the quality control of gynostemma pentaphyllum makino.2. A new method for determination of the contents of active components in gastrodia elata blume was presented by HPLC, and an ideal fingerprint of gastrodia elata blume was established. Experimental results showed that the number and shape of peaks on chromatograms of the gastrodia elata blume obtained from some different resource were basically same, but content of gastrodin as a main component was obviously different in those samples. This investigation may provide some useful information for identification of gastrodia elata blume and a basis for the quality control of gastrodia elata blume. It is very important to management of GAP and toexploitation and use of TCM.3. A new method for determination of the contents of active components in camptotheca acuminate obtained form different resources was presented by HPLC, and an ideal fingerprint of camptotheca acuminata was established by calculation of the relative retention time and similarity of main active ingredients on the chromatograms of camptotheca acuminata. Experimental results showed that the number and shape of peaks on HPLC fingerprint of the camptotheca acuminata were different, but content of camptothecin as a main component was obviously different in the different samples; the contents in leafage were higher than that in seeds, which was superior to stems. This method will be useful in the future human studies with the industrialization of camptotheca acuminata and camptothecin.4. To establish an HPLC-ECD method for determination of seven kinds of phenolic compounds in five different wine samples simultaneously, and an ideal fingerprint of wine was established by HPLC-ECD. The experimental results showed that seven kinds of phenolic compounds are separated satisfactorily and there are some good linear relationship between their concentration and peak area. When ECD was applied, the sensitivity of phenolic compounds is 4~600 times than that obtained with UV detection. The study may provide a scientific basis for the quality control and research of wine.