Fast Characterization System Based On Fiber Bundle For Luminous Intensity Of Multiple Luminescent Samples

Chemiluminescence immunoassay has the advantages of high sensitivity,non-toxicity,and simple operation,and is widely used in disease diagnosis.The de-tection of a single biomarker has the shortcoming that specificity and sensitivity cannot be balanced,while the combined detection of multiple biomarkers can im-prove the accuracy of diagnosis.However,the system for detecting the chemilumi-nescence of multiple biomarkers is mainly a lens-based optical imaging system.In this imaging system,there is a contradiction between the large field of view required for array samples and the large numerical aperture required to increase sensitivity.Because for a particular lens,the product of the aperture angle and the field of view is Lagrange-Helmholtz invariant.To solve this problem,we developed a chemilu-minescence characterization system based on fiber bundles for array samples.This system can perform simultaneous and efficient detection of multiple luminescent samples,and the gain compared to the optical imaging system has reached more than 50 times.This paper is divided into the following three chapters:The first chapter is the introduction.It mainly introduces the research back-ground,theoretical basis,and research progress of chemiluminescence immunoassay and multiplexed detection.Firstly,the research background of multiple biomarkers detection,the theoretical basis of chemiluminescence,and the principle of chemilu-minescence analysis are introduced.Secondly,common chemiluminescence systems and related research progress are introduced.Thirdly,the research progress of chem-iluminescence immunoassay and research progress of multiplexed biomarkers lumi-nescence detection technologies is introduced.Finally,the research goals and signif-icance of this paper are introduced.The second chapter is the experiment and system analysis.Firstly,we designed a lens-assisted fiber bundle system and an optical imaging system as a comparison.We used the sample array made of long-lasting luminescent materials to calibrate two characterization systems.The gain of the lens-assisted fiber bundle system compared to the optical imaging system was calculated and analyzed.Secondly,we built a di-rectly coupled fiber bundle collection system.In this system,the fiber bundle is di-rectly coupled to the sensitive surface of the CCD.We used the sample array made of long-lasting luminescence material to perform the calibration experiment,calcu-lated the gain of the directly coupled fiber bundle system compared to the lens im-aging system,and analyzed the uniformity of the light signal collected by this system.Finally,we analyzed the theoretical collection efficiency of the above three systems.The third chapter is the results and discussion.Firstly,it introduces the interface and functions of the control and process software that we authorized the software company to develop for the two systems.Secondly,it summarizes the research work and the significance of this paper.Finally,the deficiencies and improvement of this research are forecasted.