Composite Structure Color Hydrogel For Cell Sensing

Cells are the basic units that make up the structure and functions of life.The cell-based sensor with living cell as the sensitive element or research object is one of the hotspots of biosensor research.It can detect the function information of cell or analyze the properties of the test objects.Cell-based sensors have many advantages,such as rapid response,sensitive detection,environmental protection.They are widely used in biomedicine,drug development,environmental monitoring and so on.At present,most cell-based sensors require huge external equipment.A composite structure color hydrogel material has been designed,which realizes visual monitoring of cardiomyocytes without complex image processing systems.Based on the difference in cell adhesion rate of materials,the separation of functional area and cell growth area can be achieved.When cardiomyocytes beat rhythmically,the photonic crystal lattice will change,realizing the transformation from micromechanics to macroscopic optics.The composite structure hydrogel material has been integrated into microfluidic device to constructe a visual heart-on-a-chip system.The composite structure hydrogel provides a new idea in cardiac sensing,drug screening and disease modeling.The detail works are as follow:1.Preparation of polyethylene glycol diacrylate(PEGDA)hydrogel film with inverse opal structure.Based on self-assembly,silica particles formed compact nanostructures with repeating structural units.The colloidal crystal template was filled with polyethylene glycol diacrylate,and the silica nanoparticles were etched to obtain inverse opal hydrogel scaffold.The structure,optical and mechanical properties of the PEGDA hydrogel film were explored.2.Preparation of gelatin methacryloyl(GelMA)film with micro-groove structure.GelMA film with micro-groove structure was prepared by UV polymerization,and the property of hydrogel was improved by innovatively incorporating reduced graphene oxide(r GO).Micro-groove structure can induce the growth direction of cardiomyocytes,which is of great significance for cardiomyocyte function.3.Construction of PEGDA-GelMA composite hydrogels for cell sensing and integration the hydrogels with microfluidic platforms into heart-on-a-chip systems.PEGDAGelMA composite hydrogel was prepared by UV polymerization,and r GO was used to improve the property of the hydrogel.Based on the difference in cell adhesion between PEGDA and GelMA,the composite hydrogel innovatively realized functional separation.By monitoring the color change of inverse opal area,cardiomyocytes contractility and pulsation frequency were evaluated.The composite hydrogel film was integrated with microfluidic device into a heart-on-a-chip,and the feedback of the cardiomyocytes to different drugs was collected.