New Nanomaterial Platform Construction And Material Synthesis And Application Of Integrated Diagnosis And Treatment

In this article,new fluorescent probes and photosensitizers are synthesized and applied to cancer treatment.The first chapter discusses in detail the development of nanomedicine in recent years and the mechanism of photodynamic therapy.It also reviews the research in recent years and clarifies the reasons for the selection of the subject.Due to the single therapeutic effect of paclitaxel and the aggregation of perylene imide probes in water,we synthesized a virus-like medium(PTX-YMSN)based on perylene imide and the targeted functional group folate(FA),loaded with paclitaxel as a chemotherapeutic drug and has the characteristics of fluorescence imaging in the second chapter.The test results show that PTX-YMSN has high biocompatibility and strong green fluorescence under the irradiation of a 488 nm light source.The results of in vitro experiments show that PTX-YMSN has a particle size of 140 nm and has good dispersion in water.In an acidic and oxidant-rich environment,PTX-YMSN still maintains stability.The application of PTX-YMSN in Hela cells shows that PTX-YMSN can successfully kill cancer cells.Photodynamic therapy is an emerging treatment method in recent years.Most organic photosensitizers have poor solubility in water,which reduces the generation of ROS and the killing efficiency of cancer cells.Therefore,we synthesized the ZIF-8nanoplatform(AZAB@ZIF-8)based on nitrogen-doped fluoroboron dipyrrole dye(AZAB)for photodynamic therapy in chapter 3.The spectrum results show that AZAB@ZIF-8 is excited by the 808 nm light source.We used DPBF probe as singlet oxygen indicator,AZAB@ZIF-8 has higher singlet oxygen generation efficiency in acidic environment.Because AZAB@ZIF-8 has high near-infrared absorption,it can be applied to deeper tissues for photodynamic therapy.In vitro experimental results show that AZAB@ZIF-8 has good biocompatibility and high phototoxicity,indicating that AZAB@ZIF-8 can effectively kill cancer cells.