Enzymatic Synthesis Of Plant Sterol Esters Enhanced Integrated Process

Phytosterols are triterpene analogs of cholesterol,which are mainly derived from natural plants,especially oil seeds such as corn,soybeans and rapeseed.Research results show that plant sterols not only reduce the concentration of cholesterol in the blood,but also reduce the content of high-density and low-density protein combined with cholesterol,which has a good effect on the prevention of cardiovascular diseases,and has anti-inflammatory,anti-oxidant and anti-cancer effects.And promote growth and development and other functions.However,the high melting point,water insolubility and low fat solubility of phytosterols lead to very low bioavailability.After long-term research,the research group aims to catalyze the synthesis of phytosterol esters from phytosterols,which greatly reduces the melting point of sterols,while increasing its fat solubility and strengthening the availability of plant sterols.Based on previous studies,this thesis uses the recovered esterified lipase to hydrolyze vegetable oils,produce fatty acids,and replenish the esterification process.This process can produce 700 kg of fatty acids per ton of sunflower oil,and an annual output is established.Thousand tons of sunflower seed oleic acid production line;through the recovery of excess fatty acids in the esterification process,Yarrow yeast is induced to produce lipase.After optimization,the enzyme activity in 5L tank can reach 15000 U/g,and the self-produced lipase passes After diversified preparation,it can be applied to the synthesis of phytosterol esters;the three process routes of hydrolysis,fermentation and esterification are complementary to each other,forming a closed loop in pairs,and the three constitute an enhanced integrated process for the enzymatic synthesis of phytosterol esters.