Enzyme Response To Self-assembly And Dis-assembly Of Soft Matter

Enzymatic reaction is an important means of regulating the self-assembly behavior of life system,and has the characteristics of high sensitivity and time controllability,but this method is rarely used in soft matter systems.We noticed that the depletion interaction can universally and efficiently induce colloidal particle aggregation and promote the phase transition of the chromonic system.Therefore,we design enzymatic reactions to control the intensity of the depletion,thereby regulating the soft matter self-assembly and dis-assembly.This thesis mainly studies the addition of polymers to the soft matter system,the use of the depletion force of the polymer itself to induce the self-assembly of soft matter,and the regulation of the dis-assembly of soft matter through enzymatic reactions.This method provides a new idea for studying the assembly behavior of soft matter.Specifically,the research content of this paper is mainly divided into the following two parts:(1)This paper discusses using the depletion force of the polymer itself to induce the aggregation of colloidal particles,and then through the enzymatic reaction to control the intensity of depletion in the colloidal system,thereby regulating the dis-assembly of colloidal particles.We use an inverted microscope to observe the aggregation behavior in the colloidal particle system,and by adding dextranase to hydrolyze dextran,the depletion effect in the colloidal system is reduced,thereby promoting the dis-assembly of colloidal particles.This study discovered the novel enzyme-responsive self-assembly and dis-assembly of colloidal particle systems,and revealed the mechanism of self-assembly and dis-assembly of colloidal particle systems.(2)This thesis observes the dynamic morphology evolution of phase separation in the liquid crystal system through microscope,and explores the phase transition law of isotropic phase(I),nematic phase(N)and columnar hexagonal phase(C).In the polymer solution,we observed the phase transition process of the chromonic liquid crystal system.The results show that we can control the intensity of depletion in the system by changing the concentration of dextran and the concentration of dextranase,thereby effectively promoting the phase transition of the liquid crystal system.In summary,the research results not only enrich the assembly behavior of soft matter,but also have important significance for the study of the mechanism of depletion.In terms of practical application,this research has certain reference value for the understanding of soft matter assembly and function regulation.