Studies On Preparation And Properties Of PCL-based Ciprofloxacin Hydrochloride Composite Films

Nowadays,many patients face the threat of various bacterial infections after artificial joint replacement surgery.Modifying the surface of the artificial implant to inhibit the formation of biofilm is an effective prevention and treatment method,one of the methods is creating an antibacterial film consist of polymer and antibiotics,which can release drugs slowly.This article selects ciprofloxacin hydrochloride(CIP)as the core drug,polycaprolactone(PCL)as the main carrier,polyurethane(PU)and chitosan(CS)as auxiliary carriers,and sustained-release systems have been constructed on different subtracts.The methods used to make films including low-energy electron beam evaporation(EBD),emulsification cross-linking method,and solvent evaporation method.The composition,structure and morphology of films are analyzed by different characterization methods including FTIR,XPS,SEM and so on,and the antibacterial and sustained-release antibacterial properties are tested.The main works are as follows:Using EBD to deposit PCL-CIP film with mechanical mixture of PCL and CIP powders(mass ratio: 1:1)as the target.The thickness of the film is about 2 μm,and its surface is uniform and regular.Besides,there is no chemical reaction between PCL and CIP,and the drug properties of CIP has been retained.What’s more,the composite film has good antibacterial properties against Staphylococcus aureus,and its sustained release time can be up to 3 days.On the basis of PCL-CIP film,the EBD method was used to prepare PCL-CIP/PU double-layer films with different mass ratios(1:1/0.5,1:1/1,1:1/2).The process can be divided into two steps: Firstly,deposit the bottom PCL-CIP film;Secondly,deposit the top film of PU.The thicknesses are 2.23 μm,2.60 μm,and 3.00 μm respectively,and there is no obvious interface between two layers.In addition,the contents of CIP in the PU film gradually increase from the surface of the double-layer films to the interface between two layers.What’s more,due to the protective effect of the PU film,the sustained-release antibacterial properties of the PCL-CIP/PU double-layer films are stronger than that of the PCL-CIP film.In conclusion,the PCL-CIP/PU=1:1/1 and PCL-CIP/PU=1:1/2 films have better sustained-release antibacterial properties(21 days).By optimizing the formula of emulsifier(Span 80)and crosslinking agent(glutaraldehyde)in the emulsification cross-linking method,CS/CIP composite microspheres obtain uniform particle size(about 4 μm)and smooth surface.The encapsulation efficiency(EE)and drug loading efficiency(LC)is 98.28% and 15.64% respectively.The results of antibacterial experiments show that cross-linking of glutaraldehyde has no adverse effect on the antibacterial properties of CS,and the sustained-release time of CS/CIP microspheres can reach 7 days.Then,through using the solvent evaporation method,the CS/CIP microspheres are fixed on the PCL film prepared by EBD to obtain the PCL-CS/CIP microspheres film.The CS/CIP microspheres on the surface of the composite film are evenly semi-embedded or completely embedded in the matrix PCL film,and no chemical reaction occurs between them.In the antibacterial experiment,the PCL-CS/CIP microspheres film has good antibacterial activities against Staphylococcus aureus which can last for 7 days.