Analysis Of The Relationship Between The Resistance To Disinfectant And Antibiotics In Drinking Water Distribution System

Antibiotic resistance genes remaining in the water environment has become an important environmental risk issue around the world,especially antibiotic resistance gene residues in the urban drinking water system threats to drinking water biological safety.Traditional urban water supply plant water purification process has limited removal ability of antibiotic resistance genes,not only leading to the residual gene residue in the factory water,but also through the interaction of the growth ring of the pipe network,causing the risk of resistance gene pollution of pipe network,but also.Many experiments show that the resistance gene has increased after the concentration of the water supply pipe network,but the research on the resistance gene in drinking water distribution system is less.This article takes two common chlorine-resistant bacteria in the water supply pipe network as the research object.Based on the resistance genes remaining in the factory water will interact with the pipe network biofilm,the chlorine-resistant bacteria present on the biofilm are inevitably pathogenic In the case of different levels of antibiotic resistance,we explored the resistance of common chlorine-resistant bacteria in the actual water supply network to antibiotics and the abundance of antibiotic resistance genes.After PCR sequencing,it was found that sulⅡand tet B are the two most important types among the 11 tested resistance genes.After domestication,sul II in Bacillus subtilis increased the most,from 1.71×10^-6（ARGs/16s r RNA）to 1.7×10^-1（ARGs/16s r RNA）.At the same time,the tetracycline resistance gene tet B has the greatest change in Sphingomonas.From the absence of tet B before domestication to the abundance of tet B after domestication is 2.91×10^-3r RNA).In addition,the resistance of chlorine-resistant bacteria to disinfectants under the pressure of antibiotics and the growth of bacteria under different environmental conditions are also explored.For the factory water,the concentration of BDOC can be controlled at about 1.5-2.0mg/L,thereby inhibiting the water supply network.Growth and reproduction of chlorine-resistant bacteria.In addition,we also studied whether the resistance to antibiotics can spread between different populations of bacteria.After mixing the two bacteria under the pressure of tetracycline,the pure bacteria were screened out and the drug susceptibility test found that 11 and 10 antibiotic drugs were tested.Increased drug resistance.It is confirmed that there is indeed a risk of spreading the level of antibiotic resistance of bacteria.And through disinfection experiments,it was found that the effective chlorine concentration was 3mg/L.Within 2 minutes,the inactivation rate of Sphingomonas bacilli was significantly lower than that of Bacillus subtilis.About 1.05log of resistant bacteria lost their activity;when the disinfection time reached 10minutes,neither of the two bacteria was completely eliminated.Live,there are still active bacteria.When the disinfectant concentration is 4mg/L and the disinfection time is 4min,Sphingomonas is inactivated first,but Bacillus subtilis can still survive under this disinfection condition.This shows that there is a certain relationship between the resistance of chlorine-resistant bacteria to disinfectants and antibiotics.By studying the resistance of common chlorine-resistant bacteria to common disinfectants under the influence of antibiotics,this paper focuses on the analysis of the relationship between antibiotic resistance and disinfectant resistance.It is confirmed that the drug resistance will spread to pathogenic microorganisms such as pathogenic bacteria in drinking water distribution system,which provides some reference for ensuring the safety of drinking water quality in drinking water distribution system.