Study On Tissue Culture Of Two Succulent Plants From Liliaceae

Two succulent plants,Haworthia truncata and Haworthia pygmaea var.argenteo-maculosa,belong to liliaceae,which are native to Southern Africa and are loved deeply by people for their unique figures,patterns and beautiful colors.But it is not complete about the technique system of tissue culture in H.truncata and H.pygmaea var.argenteo-maculosa.By using leaves and young inflorescence as explants,this experiment studied the disinfection of explants,callus induction and proliferation,bud induction and proliferation,rooting culture,training and transplanting seedlings,and other aspects.The research explored some major factors involved in tissue culture,including the disinfection of expalnts,the effects of the hormone of the plant growth regulator on tissue culture,which would establish a foundation for H.truncata and H.pygmaea var.argenteo-maculosa to have a complete tissue culture system and industrial production.The main results were as following:（1）By using leaves and young scape as explants,added different concentration of6-BA,KT,NAA,IBA and TDZ to MS to induce the callus.The results showed that more suitable culture medium for callus induction of H.truncata leaves and H.pygmaea var.argenteo-maculosa leaves was MS+TDZ 2.0 mg·L^-1+NAA 0.5mg·L^-1;and more suitable culture medium for callus induction of H.truncata scape and H.pygmaea var.argenteo-maculosa scape was MS+6-BA 2.0mg·L^-1+NAA 0.1mg·L^-1.And much more suitable temperature for callus induction was 25±2℃.Contrasted with H.truncata leaves and H.pygmaea var.argenteo-maculosa leaves,callus induction of H.truncata scape and H.pygmaea var.argenteo-maculosa scape was much easier to induce.In terms of callus proliferation culture in the experiment,the most suitable medium for H.truncata leaves and H.pygmaea var.argenteo-maculosa leaves,as well H.truncata scape was MS+TDZ 2.0 mg·L^-1+NAA 0.05 mg·L^-1;while,MS+TDZ 2.0 mg·L^-1 was better medium for H.pygmaea var.argenteo-maculosa scape.（2）During the period of bud induction culture,the experiment showed that the most suitable medium was MS+TDZ 0.2 mg·L^-1+NAA 0.01 mg·L^-1for H.truncata and H.pygmaea var.argenteo-maculosa.For bud proliferation culture,the result showed that more suitable culture medium was MS+TDZ 0.2 mg·L^-1+NAA 0.01mg·L^-1for H.truncata leaves and scape;while moret suitable culture medium was MS+6-BA 1.0 mg·L^-1for H.pygmaea var.argenteo-maculosa leaves and scape during the period of bud proliferation.（3）Adding different concentration of NAA to the basic medium MS and 1/2MS.The result showed that the suitable culture medium for rooting was 1/2MS+NAA0.3mg·L^-1.The rooting rate was much higher in the condition that the light intensity was 2000lx than in shading.For transplanting seedlings,different transplanting substrates had great effects on the survival rating of seedling.The favorable transplanting substrate was the garden soil、humus soil and sand,which should be mixed according to the proportion2:1:1,and the average survival rating was 58.75%,which is a little higher than in the transplanting substrate of 1/2MS with sand.