| In this study,weaned pigs were selected as the study subjects,and 45 healthy 21-day-old Du×Chang×Da three-element hybrid piglets were selected and randomly divided into 3 groups.Each group had 3 replicates and 5 piglets were repeated for feeding experiments.The test period was 20 days;the control group was fed the basal diet,and the trials 1 and 2 groups were based on the previous experiment and chose to add 400 mg/kg,600 mg/kg L-theanine to the basal diet;The phenotypic indicators of weaned piglets such as growth performance,serum biochemistry,etc.Indicators related to intestinal mechanical barriers,intestinal immune barriers,and intestinal biologic barriers,were studied to investigate the scientific role of dietary L-theanine in maintaining the intestinal barrier function of weaned piglets.Assumptions.The test results showed that L-theanine can increase intestinal gut development and enhance the expression and protein expression of tight junction proteins ZO-1 and Claudin-1 in weaned piglets(P<0.05)and reduce intestinal transit.Permeability,significantly reduced the incidence of diarrhea in weaned piglets,enhanced intestinal mechanical barriers;increased intestinal microbial diversity;improved the composition of T lymphocytes in the peripheral blood of weaned piglets under stress conditions,significantly increased The content of T lymphocytes in blood(P<0.05)and s Ig A content in ileal mucosa(P<0.05)enhanced the body’s immune response;thus,the weaning stress of early weaning piglets was relieved.The addition of L-theanine to the diet did not affect the growth and physiological function of weaned piglets.Based on the results of the trial,considering the effect of L-theanine function,it was suggested that the appropriate amount of L-theanine in the diet of weaned piglets be 400 mg/kg or so. |
