Identification And Functional Analysis Of GhTT13 Related To Proanthocyanidins Transport In Brown Cotton Fiber

In this study,according to different genome-wide data,the P-ATPase family was identified,and bioinformatics analysis was conducted.The P-ATPase gene that may be involved in the transport of proanthocyanidin in brown cotton was selected.Its function was analyzed,the main results are as follows:(1)53,51,99,and 98 P-ATPase genes were identified in G.arboretum,G.raimondii,G.barbadenseand G.hirsutum,respectively.In different cottons,P-ATPases can be divided into six subfamilies,and the members of the same subfamily have the same or similar genetic structure.There is a large gap between the gene structure of different subfamily members,and in the upland cotton,P-ATPases’ function has diversity.(2)Using fluorescence quantitative analysis,we inferred five candidate genes that may be involved in the transport of procyanidins,and selected one that was the closest to the evolutionary relationship with Arabidopsis At TT13 for further study and renamed it GhTT13.(3)GhTT13 was cloned from brown cotton fiber.Its tertiary structure was similar to that of Arabidopsis thaliana AtAHA10.Transmembrane structure analysis showed that the protein was a membrane protein.Subcellular localization results showed that GhTT13 was located on the membrane.(4)The GhTT13 was transformed into wild-type Arabidopsis and the transgenic plants were obtained.DMACA staining and proanthocyanidin content determination showed that the proanthocyanidin content in the transgenic Arabidopsis was higher than that in the wild type,and the expression of some other related genes in the proanthocyanidin metabolism pathway was higher than that in the wild type,suggesting that the GhTT13 influence was The accumulation of proanthocyanidin in the seed coat of Arabidopsis.(5)Using the pollen tube pathway method to transform brown cotton and white cotton,4 and 2 transgenic cottons were obtained respectively.The expression of GhTT13 and the content of procyanidins in transgenic cotton leaves were higher than those in untransformed cotton.It was speculated that GhTT13 participated in the transport and accumulation of proanthocyanidins..