Epidemiology Investigation Of Intestinal Parasites Of Dairy Cattle,Yellow Cattle And Water Buffalo In Anhui Province

Bovine intestinal parasitic infections are more common.Intestinal parasitic infections not only affect the growth and development of cattle,but can even cause animal death,causing huge economic losses to the cattle industry.At the same time,some parasites infected with cattle have zoonotic or zoonotic potential,and cattle can become a source of infection for these parasites,which is of great significance in public health.In recent years,the cattle raising industry in Anhui Province has developed rapidly,but the prevention and control of bovine parasitic diseases is seriously lagging behind,especially the prevalence of many intestinal parasites is still blank.In view of this,this study intends to conduct a general investigation of intestinal parasites and molecular epidemiological investigations of various protozoa in the dairy cattle,yellow cattle and water buffalo in Anhui Province,in order to prevent and control the cattle parasitic diseases in Anhui Province.Some humans share the role of the protozoa propagation process and public health significance.From March to October 2018,a total of 955 large-scale dairy cattle farms(526),7 large-scale yellow cattle farms(323),and 4 large-scale water buffalo farms(106)were collected from various regions of Anhui Province.For the fresh fecal samples,the above samples were tested by conventional methods such as saturated saline floating method and water washing and precipitation method.The results showed that the total infection rate of bovine intestinal parasites in Anhui Province was 17.28%,among which the infection rate of dairy cattle was 13.50%,the infection rate of,yellow cattle 14.55%,and the infection rate of water buffalo was was 44.34%.In the dairy cattle feces samples,Amoeba,Coccidium,Trichostrongyle,Moniezia and other insect species were detected,and Coccidium,Trichostrongyle,Amoeba,Paramphistomum and Moniezia were detected in the fecal samples of the yellow cattle.In the water buffalo feces samples,Amoeba,Coccidium,Trichostrongyle,Trichuris,and Moniezia and other insect species were detected.The dominant species of dairy cattle are Amoeba,the dominant species of yellow cattle are Amoeba and Coccidium,and the dominant species of water buffalo are Amoeba,Coccidium and Trichostrongyle,and they are mixed infections.In order to understand the epidemic and distribution of Cryptoridium spp.in dairy cattle,yellow cattle and water buffalo in Anhui Province,955 fresh fecal samples were collected and their genomic DNA was extracted.All samples were tested by PCR method based on Cryptoridium spp.18s rRNA gene.Sequencing and analysis.The results showed that the total infection rate of Cryptoridium spp.in dairy cattle,yellow cattle and water buffalo in Anhui Province was 2.41%(23/955),and the infection rates of dairy cattle,yellow cattle and water buffalo were 2.09%(11/526)and 3.1%(10/323),respectively.And 1.89%(2/106).Sequence analysis showed that 23 Cryptoridium spp.positive samples obtained in this study were C.andersoni.In order to understand the infection and genotype distribution of E.bieheusi in dairy cattle,yellow cattle and water buffalo in Anhui Province,all the samples were detected,sequenced and analyzed by the PCR method based on E.bieheusi ITS gene for the extracted fecal genomic DNA.The results showed that the total infection rate of E.bieheusi in dairy cattle,yellow cattle and water buffalo in Anhui Province was 4.19%(40/955),and the infection rates of dairy cattle,yellow cattle and water buffalo were 3.04%(16/526)and 7.12%(23/323),respectively.And 0.94%(1/106)Sequencing and analysis showed that 10 E.bieheusi genotypes were obtained in this study,including 2 known genotypes J and CHN11 and 8 new genotypes AHDC1,AHYC1,AHYC2,AHYC3,AHYC4,AHYC5,AHYC6 and AHYC7.The phylogenetic tree construction showed that except for the genotype CHN11 belonging to Group 1,the other nine genotypes belonged to Group 2.In order to understand the distribution and distribution of G.duodenalis in dairy water,yellow cattle and water buffalo in Anhui Province,the above-mentioned extracted fecal genomic DNA was first tested by Giardia SSU rRNA gene PCR method and then positive for SSU rRNA gene.The samples were subjected to PCR amplification and sequencing based on the TPI gene and the GDH gene,respectively,to identify G.duodenalis aggregates.The results showed that the total infection rate of G.duodenalis,dairy cattle,yellow cattle and water buffalo in Anhui Province was 1.88%(18/955),and the infection rates of dairy cattle,yellow cattle and water buffalo were 1.14%(6/526)and 3.1%(10/323)respectively.And 1.89%(2/106).Sequence analysis showed that 12 TPI gene isolates were aggregates E1,E3 and E11,respectively,and 5 GDH gene isolates were aggregate E.In order to understand the infection status of dairy cattle,yellow cattle and water buffalo sinensis in Anhui Province,all the samples were detected by the PCR method based on the 18s rRNA gene of Blastocystis sp..The results showed that no positive samples were found in 955 samples of cattle dung.