Transcriptome Analysis Revealed Ameliorative Effect Of Probiotic Lactobacillus Johnsonii BS15 Against Subclinical Necrotic Enteritis Induced Hepatic Inflammation In Broilers

Subclinical necrotic enteritis(SNE)broadly occurs in boilers,which reduces the growth performance by causing serious economic and social problems.Following study was conducted in order to understand the molecular mechanism underlying SNE on liver inflammation and to examine the innovative prevention of Lactobacillus johnsonii under SNE.Research was based on the regulatory molecular mechanism of Lactobacillus johnsonii BS15,and its effect on liver inflammatory pathways in broiler with SNE infection.Day old one hundred and eighty(Cobb 500)broiler chickens were distributed into 3 groups were provided diet as follows:group 1 as a normal control(NC)was fed basal diet,group 2 as a SNE control(SNE experimental model)was fed basal diet,and group 3 as a LJ(preventative group+SNE experimental model)was fed on basal diet+1×10~6 cfu LJ/g of feed and reared for 28 days.Histological observation of collected liver samples was carried out to compare the liver tissues inflammation among different treatments.RNA was extracted from the liver samples and RNA sequencing was done to analyse the gene expression which was detected with the help of Quantitative real-time PCR(q RT-PCR).RNA-Seq analysis revealed altered expressions of genes involved in liver inflammatory pathway.A total number of 385 genes were found as differentially expressed(DEGs)in the liver samples that belonged to SNE group as compared to the controlled liver samples(p<0.05).Out of those 385,117 genes were down-regulated and268 were up-regulated.The DEGs related to liver inflammation between control group and SNE group or SNE and BS15 groups,included cluster of differentiation 80(CD80),Interleukin 1 beta(IL1B),Phosphoinositide 3-Kinase regulatory subunit 5(PIK3R5),Toll-like receptor 4(TLR4),Toll-like receptor 2 A(TLR2A),and proto-oncogene protein(FOS).The RNA-Seq analysis provided DEGs expression and this result was validated by q RT-PCR.Results confirmed that these genes are essential in the regulation of liver inflammation in the SNE infected chickens.Findings of current research indicated that the hepatic inflammation could be induced by SNE in broilers.Simultaneously,effects of SNE infection on liver could be subsided by improved TLRs signalling pathway with the naturally present prophylactic strategy as Lactobacillus johnsonii BS15.