Clinical Study On The Therapeutic Effect Of Marbofloxacin Injection On Sow Mastitis-Metritis-Agalactia Syndrome

There is little report about the clinical efficacy of marbofloxacin in the clinical efficacy of MMA,caused by Escherichia Coli,Staphylococcus and Streptococcus,at home and abroad.The purpose of this study was to investigate the clinical efficacy of MMA caused by Escherichia Coli,Staphylococcus and Streptococcu.Our study provided theoretical and practical references for the clinical application of marbofloxacin in MMA.The contents and results of this study were listed as followsAccording to CLSI reference,a trace double dilution method was applied to measure the minimum inhibitory concentration(MIC)of marbofloxacin in Escherichia Coli Pleuropneumonia Actinobacillus,Streptococcus,Staphylococcus aureus,non-dairy Streptococcus,Streptococcus Pneumoniae,Mycoplasma synoviae and Mycoplasma hyorhinis.Results showed that:the MICs of marbofloxacin for the eight standard bacteria were 0.015,0.008,0.125,1,1,0.5,0.5 and 0.25 μg/mL respectively.The marboloxacin showed good antibacterial activity for all the 8 strains of standard bacteria,especially for the Sternocobacter and Escherichia coli.Agar double dilution method was employed to estimate the MIC of marbofloxacin in a broth microdilution assay aganist for Escherichia Coli(40 strains),Staphylococcus Aureus(20 strains)and Streptococcus(25 strains),and the result was compared with the vitro antibacterial activity of ciprofloxacin,norfloxacin and enrofloxacin.The results as follow the MIC50 of marbofloxacin for Escherichia Coli staphylococcus aureus,and streptococcus were 8,4 and 1 μg/mL respectively,which were lower than that for norfloxacin(32,32 and 8 μg/mL)and ciprofloxacin(16,8 and 16 μg/mL),while was close to the MIC50 for enrofloxacin(16,2,and 1 μg/mL);The MIC90 for Escherichia coli,Staphylococcus Aureus,and Streptococcus were 64,64 and 16 μg/mL,separately,which were lower than that for norfloxacin(>128,>128 and 128 μg/mL)and ciprofloxacin(>128,>128 and 128μg/mL),and the result was close to that enrofloxacin(>128,32 and 32 μg/mL)We selected 150 MMA pigs from the pig farm,and then these experimental pigs were divided into high marbofloxacin-dose group,medium marbofloxacin-dose group,low marbofloxacin-dose group,enrofloxacin control group and control group.Each group had 30 pigs,using the commercially available enrofloxacin injection as a control drug,clinical efficacy test was carried on the intramuscular injection.The effective rate was significantly higher in the high dose group,middle dose group and enrofloxacin control group than that in the low dose of marbofloxacin(P<0.05),but there was no significant difference between the three groups(P>0.05).The cure rates of the high dose group,the middle dose group and the enrofloxacin control group were higher than 86%at day 4 after administration.The effective rates of the two groups were(100%),higher than the low marbofloxacin-dose group,but there was no significant difference compared with the enrofloxacin control group The results showed that marbofloxacin had good therapeutic effect for Metritis-mastitis-agalactia(MMA).