| In order to clarify the effect of the plant-derived compound sterility agent ND-1(Nongda-1)used to reduce the number of rodent populations on non-target animals,a 30 mg/kg nano-sized ND-1 sterility agent was used as the test The main components of the medicine are shikonin and quinestrol.Three yellow chickens(Gallus gallus domesticus),domestic pigeons(Columba livi),and small-tailed sheep(Ovis aries)(hereinafter referred to as sheep)were used as experimental objects.Experiments on palatability and infertility,dissect and collect blood from experimental allimals,and carry out relevant tests.The palatability test of three non-target animals on the plant-derived compound sterilizer ND-1 bait showed that sheep had better palatability on the finished sterilizer bait;the three yellow chickens refused to feed on the finished sterilizer bait.Sterility agent ND-1 bait is significantly less palatable than other chicken feed;domestic pigeons refuse to eat the finished sterile agent bait.In addition,sheep and three yellow chickens had no obvious abnormal reaction after feeding ND-1 bait.The use of 30mg/kg nano-sized ND-1 infertility agent on the infertility effect of three non-target animals showed that the organ coefficients of the three yellow chicken and domestic pig experimental groups were not significantly different from the control group.Shikonin was not detected in the blood of the three non-target animals,but it contained low concentrations of ethinyl estradiol.After testing,the concentration of progesterone in sheep blood is all>0.5ng/ml.At this concentration,sheep can be successfully conceived.Therefore,the infertility agent has no infertility effect on the above three non-target animals,and does not affect the economic value of non-target animals.The plant-derived compound sterility agent ND-1 can be safely used in the living environment of the above three non-target animals. |