Isolation And Identification Of Dominant Bacteria On The Body Surface Of Agarwood Pretreatment And Its Influence On Aroma

Aquilaria sinensis(Lour.)Gilg is a plant of the genus Aquilaria in the Aquilaria family.It is a unique medicinal plant for the production of agarwood in our country.Incense and fragrance materials are mainly distributed in Guangdong,Hainan,Guangxi,Fujian and Taiwan in China.Domestic agarwood has important clinical medicinal value and is regarded as a top-grade medicinal material.Since the massive mining of agarwood since the 1970s,the wild forest of Agarwood has been greatly damaged,leaving only scattered and scattered remaining plants.In this study,the artificial pure forest of Baimuxiang from the State-owned Forest Farm of Chengmai County,Hainan Province was used as the research object.The Baimuxiang was treated with different aroma treatments,the surface of the tree and the dominant fungi on the discolored wood were separated and purified,and the strains were identified by molecular biology.Purified bacteria are used to catalyze the Agarwood in solid state,and the strains with catalytic effect(the chemical composition of the Agarwood changes significantly before and after the catalysis)are screened,and the catalytic effect is studied by GC-MS technology.Finally,the fungus with the catalytic effect is inoculated to the Agarwood tree.In the body,the volatile oil and chemical components of agarwood substances were determined,and the influence of different fungi on the aroma of Agarwood incense was studied by GC-MS.In order to meet the rapid development of the domestic agarwood planting industry,we explored efficient aroma-forming technology and agarwood aroma-promoting fungi The expansion of the library provides a theoretical basis.The main findings are as follows;(1)Isolation and identification of dominant microorganisms on the surface of the tree After treating Agarwood with different aroma-forming methods,isolate,purify and identify the bacteria on the epidermis,damaged parts and discolored parts of Aquilaria sinensis.Two fungi were isolated and purified from the surface of the white woody balsam tree after the pretreatment of burning incense:Trichoderma sp.and Neurospora sp..After the electric shock pretreatment,24 fungi were isolated and purified from the discolored wood at the mouth of the scented wood.Among them,Fusarium sp.accounted for 16 strains,Lasiodiplodia sp.accounted for 3 strains,and Talaromyces sp.accounted for 5 strains.(2)Screening of aroma-promoting fungiA screening test for the tolerance of identified fungi to benzylacetone.The tolerant fragrant-promoting fungi are:LV,H,HLME,CX6-3(1),CX3-CK-1,CX5-1(1)-1,a total of 6 strains,namely Trichoderma sp.、Neurospora sp.、Menanotus flavoliven、Fusarium sp.、Lasiodiplodia sp.、Talaromyces sp.The white wood shavings were used as the culture substrate,and the identified fungi were used for solid catalysis.The strains with catalytic effects(significant changes in the chemical composition of the white wood before and after the catalysis)were screened,and the catalytic products were analyzed by GC-MS.Significant changes in the chemical composition of white wood are:Trichoderma sp.、Neurospora sp.、Menanotus flavoliven.Some changes in the chemical composition of white wood before and after catalysis are:Trichoderma sp.、Neurospora sp.、Menanotus flavoliven、Fusarium sp.、Lasiodiplodia sp.、Talaromyces sp.(3)Study on the aroma-promoting fungi inducing the aroma-forming of white wood The screened strains were used to promote the aroma-setting test of the Aquilaria sinensis(Lour.)Gilg.Through the determination of the volatile oil and the chemical composition analysis of the scented woods of the Aquilaria sinensis(Lour.)Gilg,it was concluded that:Trichoderma sp.、Neurospora sp.、Menanotus flavoliven are in 6 Months can effectively induce the fragrance of Aquilaria sinensis(Lour.)Gilg.