| As one of the main production areas of muskmelon,Xinjiang ranks first in the planting area and output in China.Xinjiang has a vast area,accounting for 1/6 of China’s area.The climatic conditions in different producing areas are quite different,which leads to the obvious difference in the flavor and quality of the same variety of melons in different producing areas.However,there are few studies on the identification of key differential genes and aroma components in melon aroma formation from different producing areas.Therefore,this paper takes’ Nasmi ’melon as the research object and obtains the two producing areas with the largest difference by analyzing the difference of fruit quality in the main producing areas of Xinjiang.Then transcriptome sequencing and gas-phase ion migration spectroscopy were used to compare the different genes and aroma components related to the aroma of ’Nasmi’ melon from the two producing areas,to identify the differences of key genes and main aroma components in the aroma formation of ’Nasmi’ melon from different producing areas.The research can provide the theoretical basis for the construction of the Xinjiang muskmelon brand and precise breeding.The main research results are as follows:1.By measuring the quality indexes of ’Nasmi’ in Altay,Kashi,and Turpan,it was found that most of the quality indexes showed significant differences among the three regions,most of which were the highest in TT1,and the citric acid content in KT1 was better than that in AT1 and TT1.AT1 had the best scavenging rates of fumaric acid,ABTS,and DPPH free radicals.According to the membership function analysis,TT1 and AT1 had the greatest difference in melon quality.Therefore,these two producing areas were selected for the next experiment.2.Transcriptome sequencing analysis of AT1 and TT1 showed that 9658 genes were differentially expressed between the two regions,4737 genes were down-regulated and 4621 genes were up-regulated;The GO analysis of these differentially expressed genes revealed that 7163 differentially expressed genes were annotated into multiple GO entries under three major classifications.By KEGG annotation of the differentially expressed genes,a total of 4061 differentially expressed genes were enriched in 136 metabolic pathways,among which there were more differentially enriched genes such as RNA transport and endocytosis.Through Pathway-analysis of aroma-related pathways,the key differential genes for melon aroma formation in different regions were identified as ethanol dehydrogenase,3-hydroxyl-Co A dehydrogenase,acyl-Co A oxidase,long-chain acyl-Co A synthase,acetaldehyde dehydrogenase,acetyl-Co A acyltransferase,and so on.3.Eleven differentially expressed genes were randomly selected and verified by real-time quantitative PCR,which showed that the transcriptome sequencing results were consistent with them,indicating that the transcriptome sequencing data was accurate.4.By comparative analysis of the aroma components of the same melon ’Nasmi’ from two producing areas,it was found that there was no difference in the types of aroma components of melon from the two producing areas,but there was a significant difference in the contents of each component.The main aroma components of melon from two producing areas were esters,alcohols,and aldehydes.Among them,the total content of aldehydes in Turpan(TT1)is much higher than that in Altay(AT1),especially E-2-nominal,5-methyl-2-thiophene formaldehyde,and nonaldehyde.The total aroma content of the TT1 sample is 1.7 times higher than that of the AT1 sample,which preliminarily indicates that the aroma content of TT1 is better than that of the AT1 sample. |
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