Study Of Allicin On Reproductive Regulation Against Male Sitotroga Cerealella

Sitotroga cerealella is a worldwide stored-grain pest.Interfering its’reproductive behavior can control its’reproduction effectively to reduce the harm to grain caused by the moth.The effect of allicin(LC₁₀)on male’s sperm and accessory gland proteins（MAGPs）of Sitotroga cerealella was tested by observing the structure of the internal reproductive system and sperm morphology of male in this research.Meanwhile,the effect of DT-MAGPs on female reproductive behavior,such as mating behavior,courtship behavior,and reproductive capacity was also performed by micro-injection.The main results of this research are as follows:1.The influence of allicin on morphology and quantity of sperm was clearly clarified.The sperm in the copulatory pouch existed in a single free state,a"line"type and there were two types of sperm:eupyrene and apyrene.The genetic material located in the head of eupyrene,while the latter was absent,and the length of the eupyrene was significantly longer than that of the apyrene（p<0.001）.The number of eupyrene,apyrene and total sperm in the female copulatory pouch decreased significantly after mating with the male（DT）（p<0.01）.2.Internal reproductive system of male was observed and the accessory gland protein content of the male（DT）was also tested after mating.The results indicated that male internal reproductive system is composed of permary,vas deferens,ejaculatory duct,seminal duct,and accessory gland.The accessory gland was one pair of tubular glandular tubes.The content of accessory gland protein of the male treated with DT decreased significantly either pre-mating or post-mating（p<0.05）.At the same time,the results showed that MAGPs cause mating refractoriness of females,and restrain recopulation.The first mating peak and the mating start time of female were significantly delayed after the injection of MAGPs,the courtship rate of the first day reduced significantly,and peak of the courtship peak was earlier（p<0.05）.3.The indirect regulation effect of allicin on the reproductive ability of female was primarily studied.DT-MAGPs were injected into the untreated female.The results showed that the first mating rate and duration reduced significantly after mating with untreated males（p<0.01）and the first number of the zygote decreased ultimately（p<0.05）;also,its lifetime and the total number of the zygote decreased significantly（p<0.05）.In addition,high concentration of male accessory gland proteins（H-MAGPs）could significantly reduce female’s lifetime（p<0.01）,so did mating itself（p<0.01）.This phenomenon may result from the MAGPs.This study showed that the allicin can reduce population of the moth by decreasing the number of sperm.It also verified that DT-MAGPs regulate the reproductive capacity of female.All results mentioned above will be helpful to further clarify the regulation mechanism of allicin,also provide the theoretical basis for the rational use of botanical insecticide and insect behavior regulation.