Effects Of Transgenic Cry1Ac/SCK Rice Grain On Larvae And Internal Major Bacteria Of The Sitotroga Cerealella

The comparative study of the impact of transgenic CrylAc/SCK rice grain and non-transgenic rice grain parent on growth, development, reproduction, important detoxifying enzymes and other major physiological and biochemical indicators of the Sitotroga cerealella of stored-grain insects. Besides, based on the separation and purification of internal major bacteria of the larvae of Sitotroga cerealella, this paper reports biological activity effects of Cryl Ac protoxin on internal major bacteria of the Sitotroga cerealella. The main findings are as follows:1Effects of transgenic CrylAc/SCK rice grain on growth, development and reproduction of the larvae of Sitotroga cerealellaCompared with non-transgenic rice grain control, emergence rate, adult longevity and oviposition number of per female of Sitotroga cerealella which fed on transgenic CrylAc/SCK rice grain were significantly decreased, and larvae-pupae period were significantly prolonged. Among them, the emergence rate and oviposition number of per female of decreasing range was40%and7.2eggs separately, and larvae-pupae period could put off8.10days. However, adult longevity, egg period of the first generation and hatching rate lower than that in control group, but there was no significnt difference.2Effects of transgenic CrylAc/SCK rice grain on important detoxification enzymes of the larvae of Sitotroga cerealellaThe activity of acetylcholinesterase in larvae fed on transgenic CrylAc/SCK rice grain for24,36,48,60and72h were higher than that of the control fed on the transgenic CrylAc/SCK rice grain except12h, where the enzyme activity reached significant differences for36h and60h and highly significant difference for24h and72h. Besides, Carboxylesterase activity were significantly higher than the control, where the enzyme activity were highly significant differences for24h,36h,48h and72h. But, acid phosphatase and alkaline phosphatase activity were lower than the control, acid phosphatase activity were significantly lower than the control for60h and72h, alkaline phosphatase activity were significantly lower than the control except12h, where the enzyme activity were highly significant differences for36h,48h and72h. However, Glutathione-S-transferase enzyme activity were no significnt difference except12h and24h. So the results show that acetylcholinesterase, carboxylesterase and alkaline phosphatase of these detoxifying enzyme play important detoxification function in CrylAc/SCK toxic protein metabolism, while the acidic phosphatase and glutathione-S -transferase enzyme probable have aless important effect.3Effects of transgenic CrylAc/SCK rice grain on important antioxidant system of the larvae of Sitotroga cerealellaThe O2·content in larvae fed on transgenic CrylAc/SCK rice grain for12,24,36,48,60and72h were lower than that of the control fed on the transgenic CrylAc/SCK rice grain except24h, where the O2· content reached significant differences for48h and72h, and reached the lowest at72h. Besides, superoxide dismutase(SOD) activity were significantly higher than that of the control for12h,24h,36h and60h, where the enzyme activity reached highly significant differences for12h and36h. But, Peroxidase(POD) activity were significantly lower than that of the control for12h,24h,36h and60h, where the enzyme activity reached highly significant differences for12h,24h and60h, and was0.47times of the control at60h. However, catalase(CAT) activity were higher than the control except48h, where the enzyme activity reached significant differences for24h and60h, and highly significant differences for12h. Hence the results show that Superoxide dismutase (SOD) and catalase(CAT) activity play an important role in antioxidant system, but Peroxidase(POD) activity was inhibited after the larvae of Sitotroga cerealella fed on transgenic CrylAc/SCK rice grain.4Determination of toxicity of CrylAc protoxin on the newly hatched larvae of Sitotroga cerealellaDetermination of toxicity of standard sample and extraction of CrylAc protoxin showed that toxic regression equation on the newly hatched larvae of Sitotroga cerealella in laboratory test separately as follows:y=2.755x-.208, R2=0.971and y=3.069x-0.460, R2=0.947. Besides, determination of LC50of standard sample and extraction of CrylAc protoxin came to the conclusion that LC50was1.190ug/mL and1.412ug/mL separately. Thus, the results show that the activity of extraction of CrylAc protoxin is weaker than that of standard sample, and lay a foundation for effect of extraction of CrylAc protoxin on bacteria in vivo instar larvae of Sitotroga cerealella.5Effect of extraction of CrylAc protoxin on bacteria in vivo instar larvae of Sitotroga cerealellaThe cultivate major bacteria from the vivo2nd instar larvae of Sitotroga cerealella was a total of six families and11species, and belonged to Vibrionaceae1species, Bacillus1species, Enterobacteriaceae3species, Micrococcus Division1species, Neisseria Division2species and the Pseudomonas Division1species. Then, the results of effect of extraction of CrylAc protoxin on these bacteria showed that the bacterial inhibition diameters were almost zero by the inhibition zone method, and had a less influence by denaturing gradient gel electrophoresis (DGGE). So the results showed that the CrylAc protoxin have almost no inhibitory effect for cultivate major bacteria.