Mining Related Genes Of Tomato Short Internode Formation

1.Tomato(Solanum lycopersicum L.)is one of the widely cultivated horticultural crops in the world.The expansion of the planting area,gradually decreasing of the cultivated land area and the structural shortage of labor supply contradictions are increasingly prominent.Small and compact ideal plant type in tomatoes can show the advantages such as increasing planting density,improving lodging resistance,reducing the cost of production,suitable for saving effort cultivation,which have great significance to improve the efficiency of the tomato cultivation.However,research on the developmental regulation of internode length in tomato,especially the formation of short internodes,is very limited.Therefore,in order to parse the molecular regulation mechanism of internode shorter,and the application of ideal plant type,twocultivated tomato lines ‘CH’ and ‘DH’,with different internode length,were used as the materials.The phenotypic characterization,physiological and biochemical analysis,cytology observation,comparison the transcriptome analysis and candidate genes of bioinformatics analysis methods were utilized for in-depth researching.The major results are as follows:1.By measuring the length of the internodes in 6 periods and drawing the growth dynamic graph,two materials had no significant differences in plant height and internode length except the stem thickness at the period of T25;the two materials had no significant differences in the internode length,both plant height and stem thickness showed extremely significant differences at the period of T35.Through cytological observation,the short internode tomato ’CH’ compared with the normal internode tomato ’DH’ showed a shorter cell length and a smaller cell area.Through the analysis of the physiological and biochemical indicators of the two materials in the two periods,it was found that,except for the IAAO in the T25 period,the other measurement indicators had extremely significant differences.2.Transcriptome sequencing was performed on the stem segments of T25 and T35,and a total of 77.36 Gb of Clean Data were obtained.After annotating and analyzing,the differentially expressed genes were mainly involved in the signal transduction pathway of plant hormones.Through further analysis of this pathway,4(GID1B,GH3.10,ARR3 and IAA13)maight be involved in the related genes that regulated the formation of tomato short nodes.3.The bioinformatics and spatiotemporal expression pattern of the GH3.10 gene indicated that the GH3.10 gene encoded a protein containing 600 amino acids,which was predicted to be located in the cytoplasm.The secondary structure of the protein showed that it contained 40.83 % of α.Spiral,5.17 % β-sheet,39.33 % random coil;homology comparison analysis certified that the protein belonged to the same branch as eggplant,the homology relationship was the closest,and the homology relationship with cucumber and corn was the farthest.The spatiotemporal expression results showed that GH3.10 was expressed in different tissues of tomato ’CH’ in short internodes,but the expression was highest in the leaves,followed in the stem,and the lowest in the root.