Effects Of Dietary Nisin Supplementation On Growth Performance,Antioxidant Ability,Immune Function And Intestinal Health In Broiler Chickens

Due to the ban on antibiotic growth promoter(AGP),the broiler breeding industry and the feed industry urgently need to develop safe,efficient,and non-ecologically hazardous alternatives to AGP.Nisin is a peptide antibacterial substance composed of 34 amino acids,which has an inhibitory effect on most Gram-positive bacteria.Practical applications in the food and pharmaceutical industries have shown that Nisin has good antibacterial effects,is not easy to produce drug resistance,and does not produce no drug residues,suggesting that Nisin has the potential to become a substitute for AGP.The appropriate Nisin dosage was selected by broiler production performance and slaughter performance,and its effects on meat quality,antioxidant and immune function,intestinal health were detected.And the feasibility of Nisin application in no AGP diets was comprehensively evaluated,which will provide theoretical support for the application of Nisin in no AGP diets for broilers.Exp.1 Effects of dietary Nisin supplementation on growth performance,slaughter performance and meat quality in broiler chickensIn this experiment,360 healthy Hubbard roosters with similar body weights(38.40 ±0.8 g)were selected at the age of 1 day.A single factor completely randomized design was adopted and randomly divided into 3 groups with 6 replicates in each group and 20 chickens in each replicate.The control group was fed basal diet,and the experimental group was fed with 0.08% and 0.11% Nisin in the basal diet,respectively.The results showed: 1)Compared with the control group,the addition of 0.08% and 0.11% Nisin to the diet significantly reduced the ADFI and 22-42 d F/G of broilers on 22-42 d and 1-42 d(P <0.05).2)Addition of Nisin in diet has no significant effect on slaughter performance(P> 0.05);3)Addition of0.08% and 0.11% Nisin in diet has no significant effect on breast and leg muscle quality,amino acid composition,fatty acid composition,moisture and ash content(P> 0.05).The results of this experiment show that adding 0.08% and 0.11% Nisin to the diet can improve the growth performance of broilers.Exp.2 Effects of dietary Nisin supplementation on antioxidant ability,immune function and intestinal health in broiler chickensSame with experiment 1.The results showed that: 1)At 21 days of age,the total antioxidant capacity(T-AOC)of the Nisin group was significantly higher than that of the control group(P<0.05);at the age of 42 days,the serum superoxide dismutase(T-AOC)of0.11% Nisin group-SOD)content was significantly higher than the control group(P<0.05).2)Compared with the control group,the addition of 0.11% Nisin in the diet significantly increased the serum immunoglobulin M(Ig M)content of the 21-day broiler chickens(P<0.05);the 0.08% and 0.11% groups significantly increased the 42-day broiler serum immunity Globulin G(Ig G)content(P <0.05).3)The height and V/C of jejunum villi in the21-day and 42-day-old broilers in the 0.11% Nisin group were significantly higher than those in the control group(P<0.05).4)Compared with the control group,the addition of 0.08%and 0.11% Nisin in the diet significantly increased the 21-day-old jejunum secretory immunoglobulin A(s Ig A)content of broilers;the s Ig A content of the 42-day-old broilers in the 0.11% group significantly increased(P <0.05).The addition of 0.11% Nisin to the diet significantly reduced the serum DAO content of broilers at 21 and 42 days(P <0.05);the serum DAO content of broilers in the 0.08% group significantly decreased at 42 days(P<0.05).The results of this experiment show that the addition of Nisin to the diet can improve the antioxidant and immune performance of broiler chicken serum,improve the intestinal immune barrier function,and promote the development of small intestinal villi.