Standardization Of Seed Inspection And Key Techniques Of Bionic Cultivation Of Trollius Chinensis

Trollius chinensis Bunge.Is a kind of multi-functional plant,which is widely used in medicine,flowers,food,ecological restoration and so on.It has good medicinal value,ornamental value and ecological value.The seed inspection methods of Trollius chinensis were systematically studied,and the seed inspection regulations were formulated;The physiological and biochemical changes of Trollius chinensis seeds during germination were analyzed;And the key technology of bionic cultivation of Trollius chinensis was studied.The results are as follows1.The seeds of Trollius chinensis were oval,round,obovate and fusiform in shape;There are five colors: black,yellow brown,green brown,gray and brown;The range of size is 1.59-1.87 mm in length,0.88-1.18 mm in width and 0.81-0.91 mm in thickness.2.The minimum weight of sample for seed inspection of Trollius chinensis is 250 G.The weight of 1000 grains was 0.8000-1.1000g;The germination rate was 88.7% when the seeds were soaked in 550 mg / L gibberellin for 13 h,which was significantly different from CK treatment(P < 0.05);The suitable germination temperature is 20 ℃;The suitable germination bed is paper germination;The first count was on the 8th day and the last count was on the 15 th day;The best method of seed viability test was bromothymol blue(BTB)method,and the culture time was 2 h;In the moisture determination,the high temperature constant temperature grinding method was used to dry for 3 h.3.Gibberellin can effectively break the dormancy of Trollius chinensis seeds,promote the production of soluble sugar and reducing sugar,and promote the continuous degradation of starch,which can significantly improve the seed quality α-Amylase β-Among the activities of amylase and peroxidase,550 mg / L gibberellin had the best promotion effect,which played a positive role in breaking seed dormancy and promoting seed germination.4.The results showed that the order of emergence rate of Trollius chinensis was greenhouse flat bed > non-woven bag > open bed > plastic bag;The emergence rate of different soil thickness was 1.0cm > 0.5cm > 1.5cm > 2.0cm.5.Under the bionic cultivation condition of Trollius chinensis on natural grassland,the transplanting time had an effect on the survival rate of seedlings,and the order was dormancy period > withered yellow period > turning green period > nutrition period > flowering period;The transplanting density also had an effect on the survival rate of seedlings,and the order was 35 cm × 40cm>30cm × 40cm>40cm × 40cm。...