Effect Of Adding Tartaric Acid To Diet With Different Concentrate To Roughage Ratio On Digestion,Metabolism And Rumen Flora Diversity Of Sheep

In this paper,the effect of adding tartaric acid to diet with different concentrate to roughage ratio on digestion,metabolism and rumen flora diversity of Aletai ewes was studied.Ten healthy Aletai ewes about 1 year old,with body weight of(40.43 ± 1.70)kg were selected and randomly divided into 2 groups(5 in each group).The experiment was carried out in two phases.The first phase of the experiment was fed diet with concentrate-to-rough ratio of 5:5,the control group and the experimental group were added with DL-tartaric acid 0 g/d/head and 2 g/d/Head respectively.The phase 2 was fed diet with concentrate-to-rough ratio of 3:7,the control group and the experimental group were added with DL-tartaric acid 0 g/d/head and 2 g/d/Head respectively.The results were as follows:(1)Under the diet with concentrate-to-rough ratio of 5:5,compared with the control group,adding with DL-tartaric acid 2 g/d/head,the apparent digestibility of DM,OM,CP,and energy of sheep was decreased by 1.99%(P>0.05)and 3.31%(P>0.05),2.9%(P>0.05),-1.2%(P>0.05).Apparent digestibility of ADF and NDF increased by 0.31%(P>0.05)and 2.91%(P>0.05).Apparent digestibility of calcium and phosphorus decreased by 4.68%(P>0.05)and-14.98%(P>0.05);nitrogen deposition and deposition rate reduced by 10.39%(P>0.05)and 2.97%(P>0.05).Under the diet with concentrate-to-rough ratio of 3:7,compared with the control group,adding with DL-tartaric acid 2 g/d/head,the apparent digestibility of DM,OM,CP,and energy of sheep was increased by 13.85%(P<0.05),15.03%(P<0.05),23.16%(P>0.05),13.63%(P<0.05).Apparent digestibility of ADF and NDF increased by 46.63%(P<0.05),38.62%(P<0.05).The apparent digestibility of calcium and phosphorus increased by 2.01%(P>0.05),14.01%(P<0.05).Nitrogen deposition and deposition rate increased by 23.45%(P>0.05)and 23.9%(P>0.05).(2)The top four dominant bacterial groups in rumen fluid of the four groups are sorted in order from Bacteroidetes,Firmicutes,Proteobacteria,and Spirochaetes.At the genus level,the rumen bacteria which proportion was greater than 1%are unclassified Prevotaceae,unclassified Bacteroides,unclassified rumenaceae,unclassified phytobacteriaceae,Methanobrevibacter and Unclassified Lachnospiraceae.under the diet with concentrate-to-rough ratio of 3:7,adding with DL-tartaric acid 2 g/d/Head,unidentified Bacteroidales,the level of unidentified Lachnospiraceae in rumen fluid were increased significantly(P<0.05),while the Methanobrevibacter was decreased significantly(P<0.05).In conclusion:(1)Under the diet with concentrate-to-rough ratio of 5:5,compared with the control group,adding tartaric acid 2 g/d/Head to diet had no significant effect on apparent digestibility,nitrogen deposition,and deposition rate,(2)Under the diet with concentrate-to-rough ratio of 3:7,compared with the control group,adding tartaric acid 2 g/d/Head to diet increased the apparent digestibility,nitrogen deposition,and deposition rat significantly.(3)Under the diet with concentrate-to-rough ratio of 3:7,adding tartaric acid 2 g/d/Head,unidentified Bacteroidales,the level of unidentified Lachnospiraceae in rumen fluid were increased significantly,while the Methanobrevibacter was decreased significantly.