The Identification And Functional Study Of Gene CsLAZY1 Regulating Branch Angle In Tea Plant (Camellia Sinensis)

The tea plant is one of the important economic crops in China,Non-alcoholic beverages made from its leaves are welcomed by consumers.In recent years,scholars at home and abroad have studied tea plant mainly in the aspects of taste,processing,disease resistance and variety breeding,but there are few studies on tea plant types.In this study,three LAZY genes were identified by genome-wide analysis,and their structure,expression pattern and phylogeny were analyzed by bioinformatics,by using real-time fluorescence PCR(q RTPCR),the expression differences of LAZY1 in different tea plant tissues and varieties were analyzed,and subcellular localization and transgenic verification were carried out.The main results are as follows:1.The structure of CsLAZY2 is quite different from CsLAZY1 and CsLAZY3.Through cluster analysis showed that CsLAZY1,3 and At LAZY1,Os LAZY1 and Pt LAZY1 were clustered in the same subgroup.By analyzing the transcriptome of eight different organs in the tea plant,Only CsLAZY1 was specifically expressed in the stem,which was consistent with its function of controlling branching Angle,while CsLAZY2 and CsLAZY3 were highly expressed in leaves and flowers,respectively.The analysis of promoter elements showed that CsLAZY1 contained more hormone response elements,CsLAZY3 contained more light response elements,while CsLAZY2 contained less response elements.2.The expression pattern of CsLAZY1 was analyzed by q RT-PCR.The results showed that CsLAZY1 was highly expressed in stems of most varieties,followed by leaves and buds,but not in roots.the expression level of CsLAZY1 in batch-type tea plants was significantly higher than that in upright tea plants.The expression of CsLAZY1 is related to the branching angle of tea plants.3.By constructing the expression vector of CsLAZY1-GFP fusion protein,and transferring it into protoplasts of tobacco and Arabidopsis thaliana,subcellular localization analysis showed that CsLAZY1 was mainly located on the cell membrane.4.When CsLAZY1 was overexpressed in Arabidopsis thaliana,it was found that CsLAZY1 transgenic plants responded to gravity more quickly than wild-type Arabidopsis thaliana plants,which indicated that CsLAZY1 could further regulate the branching angle of tea plants by responding to gravity.