Study On The Mechanism Of ZmPP2C6 Regulating Maize Drought Response

Zeamays is one of the most important feed crops in the world.Drought stress is one of the main abiotic stresses in nature.ABA not only regulates plant development,but also plays an important role in regulating plant response to various environmental conditions.Many studies have shown that ABA synthesis can be promoted under adverse conditions such as drought,salt and low temperature,and the expression of downstream genes can be regulated through signal transduction to participate in the stress response.However,there are few studies on drought resistance mechanism involving ABA in maize,so it is of great significance to study the molecular mechanism of ABA in regulating drought response in maize.It has been reported that HAI1,AIP1 and HAI3 are associated with water loss and drought in Arabidopsis thaliana.In this study,homologous genes ZmPP2C6 of HAI1,AIP1 and HAI3 in maize genome were screened out through bioinformatics analysis.ZmPP2C6 gene was cloned from maize B73,and the molecular mechanism of ZmPP2C6 gene involved in maize drought resistance was preliminarily explored,and it was found that ZmPP2C6 was involved in maize drought resistance response.The following are the main research results of this subject:1.Bioinformatics analysis:The PP2C family of Arabidopsis thaliana was used for comparison,and it was found that there were 16 PP2C genes highly homologous to Arabidopsis thaliana in maize,which were named ZmPP2C1-16.2.Tissue expression pattern analysis showed that ZmPP2C6 gene was mainly expressed in rhizomes and leaves.3.According to the sensitivity experiment of mutants to ABA during germination,the mutant zmpp2c6 was more sensitive to ABA than the wild type under 25μM ABA treatment,which revealed that this gene may improve plant tolerance by participating in aba-mediated signal transduction pathways.4.Under drought stress,the mutant was more drought tolerant than the wild type.The content of proline and hydrogen peroxide in the mutant was significantly higher than that of the wild type,and the water loss rate of the mutant was slower than that of the wild type,which was consistent with the drought tolerance phenotype of the mutant.5.Subcellular localization showed that ZmPP2C6 protein was localized in the nucleus.6.The bimolecular fluorescence complementary shows that ZmPP2C6 interacts with SnRK2.5 and SnRK2.10 respectively in the nucleus.These results indicate that ZmPP2C6 may participate in drought regulation mechanism together with SnRK2.5 and SnRK2.10.In Summary,ZmPP2C6 is involved in maize drought resistance response and may enhance plant tolerance by participating in ABA-mediated signaling pathways.