Optimization Of Culture Conditions And Omics Of Medicinal Function Of Phylloporia

Phylloporia,belonging to Hymenochaetaceae,Hymenochaetales,Agaricomycetes,Basidiomycota,is a genus of wood-inhabiting macrofungi,and has been used as traditional medicines in folklore.Recently,Phylloporia has been frequently recorded in China,and of this genus P.pulla and P.fontanesiae are recognized as two main medicinal species.Although the medicinal functions of Phylloporia have been recognized in certain medicinal studies,the current understanding is far from enough to support its development in commercial production.In this study,through single factor experiment,PB experiment,steepest climbing experiment and three factor-three level response surface experiment,the medium nutritional components of Phylloporia in liquid fermentation were optimized.The optimal medium was simulated based on six components,viz.potato-carrot,glucose,peptone,KH₂PO₄,Mg SO₄?7H₂O and Zn SO₄?7H₂O.On this basis,this study realized the construction of c DNA library and paired-end sequencing through the second-generation sequencing technology based on Illumina sequencing platform,sequenced and analyzed the sample metabolome through UHPLC-Q-TOF-MS,integrated and screened the sequencing data by bioinformatics means,and annotated the sample transcriptome and metabolome data based on GO and KEGG databases.The significantly differential transcriptomes and metabolites of the two species of Phylloporia on the 1st,4th,7th and 10th day of fermentation were analyzed.During the dynamic monitoring and analysis of the growth process of the two species of Phylloporia,it was found that the expression of genes and metabolites related to mycelial growth and development was the most active in the medium fermentation culture for P.pulla,while the differential expression of genes and metabolites was mostly concentrated at the late stage of fermentation culture for P.fontanesiae.In addition,the transcriptomic and metabolomic data also revealed the changes of medicinal secondary metabolites and their related transcripts at different growth stages of Phylloporia.Through the screening of key medicinal secondary metabolites and the joint analysis of transcription and metabolism,the trend of changes in secondary metabolites and genes with fermentation time was revealed.Moreover,the pathways related to the synthesis and regulation of medicinal secondary metabolites of Phylloporia,viz.Steroid biosynthesis,Terpenoid backbone biosynthesis,Sesquiterpenoid＆triterpenoid biosynthesis and Ubiquinone＆other terpenoid-quinone biosynthesis were identified.Furthermore,fifteen genes related to triterpenoids and two genes related to sterols were determined.The above results confirm the medicinal value of P.pulla and P.fontanesiae,further supplement the omics data for functional research of Phylloporia,and lay a foundation for the development and utilization of medicinal functions of Phylloporia.