| Objective:To detect the frequency of haptoglobin（Hp）genotypes and allele distributions in patients infected with Hepatitis B Virus（HBV）,analyze the association between genetic polymorphisms and genetic susceptibility to HBV infection,and Further explore the relationship between Hp genotype and clinical outcome of HBV infection.Methods:221 patients with HBV infection who met the inclusion criteria and were treated in the hospital of Jilin Provincial People’s Hospital from March 2019 to December2019 were randomly selected.According to the clinical diagnosis,121 cases were divided into asymptomatic carrier group,41 cases in HBV-liver cirrhosis（HBV-LC）group,27 cases in HBV-Hepatocellular Carcinoma（HBV-HCC）group,and Including 32 cases with HBV combined with Nonalcoholic Fatty Liver Disease（NAFLD）.According to the results of hepatitis Be antigen（HBe Ag）and Hepatitis Be Antibody（HBe Ab）,the results were divided into HBe Ag（+）/HBe Ab（-）group and HBe Ag（-）/HBe Ab（+）Group two groups,37 cases and146 cases,respectively.Fifty healthy subjects were selected as the control group.Sequence-specific Primer Polymerase Chain Reaction（PCR-SSP）was used for genotyping test to analyze the distribution of Hp1-1,Hp2-1 and Hp2-2 genotypes in patients with HBV infection.Gene counting calculates the allele frequency.SPSS 21.0 statistical software was used.Two independent sample T tests were used to compare the normal distribution measurement data between the two groups.The genotype distribution and allele frequency were compared using the Chi-square test.P<0.05 indicated statistical significance.The genotypes of the experimental group and the control group were tested by HWE’s law for the population representativeness of the samples studied.P>0.05 indicated that the samples selected for the experiment were representative of the population.Results:The genotype distribution and allele frequency of the control group and the experimental groups were calculated.The results showed that:1.In the control group,HBV combined with NAFLD,asymptomatic HBV carryover,HBV-LC,and HBV-HCC,the Hp1-1,Hp2-1,and Hp2-2 genotypes accounted for 4.00%,40.00%,56.00%;9.40%,43.80%,46.90%;11.51%,51.33%,38.94%;21.95%、41.46%、36.59%;22.22%、40.74%、37.04%.In the control group,HBV combined with NAFLD,asymptomatic HBV carryover,HBV-LC,and HBV-HCC allele Hp1 and Hp2 frequencies were 24.00%,76.00%;31.25%,68.75%;36.78%,36.78%,63.22%;42.68%,57.32%;42.59%,57.41%.Except for the HBV combined with NAFLD,the other three groups were significantly different from the control group（P<0.05）.2.A pairwise comparison of the three pairs of the HBV asymptomatic group between the HBV-LC group,the HBV asymptomatic group between the HBV-HCC group,and the HBV-LC group with the HBV-HCC group showed the genotype distribution.There was no significant difference in allele frequencies（P>0.05）.3.In the HBe Ag（+）/HBe Ab（-）group and the HBe Ag（-）/HBe Ab（+）group,the Hp1-1,Hp2-1,and Hp2-2 genotypes accounted for 5.41%,54.05%,40.54%;7.53%,46.58%,45.89%.In the HBe Ag（+）/HBe Ab（-）group and HBe Ag（-）/HBe Ab（+）group,allele Hp1 and Hp2 frequencies were 32.43%,67.57%;30.82%,69.18%.The results suggested that there was no significant difference in genotype distribution and allele frequency between the HBe Ag（+）/HBe Ab（-）group and the HBe Ag（-）/HBe Ab（+）group（P>0.05）.Conclusions:1.Hp1-1 genotype and allele Hp1 are significantly associated with genetic susceptibility and clinical outcome of HBV infection.2.There is no significant correlation between Hp genotype and HBe Ag positive,that is,it is not associated with virus replication.Hp genotype does not promote the development of patients from occult infection to liver cirrhosis and liver cancer by promoting large amounts of virus replication.3.Hp genotype distribution and allele frequency have no significant correlation with HBV combined with NAFLD. |
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