Studies On The Quality Control And Pharmacokinetics Of Callicarpa Nudiflora

Callicarpa nudiflora Hook is a famous region drug of Hainan Province,which was contained in 1977 edition of Ch P.The leaves,which can be used as medicine,show a variety of pharmacological effects,such as antibacterial,hemostatic,antiphlogistic,detoxify,stasis detumescence,carminative dampness and enhanced immunity.In the present study,the methods of quality control of Callicarpa nudiflora Hook and pharmacokinetic of active constituents were diseussed in detail.According to the Chinese Pharmacopoeia(1977 edition)and related standards,methods of traits observed,microscopic identification,moisture,total ash,acid insoluble ash,and water-soluble extract were rechecked.A reasonable revision about index description and limit mentioned above was made combining with the measured results.The TLC method of Callicarpa nudiflora was established,and the assaying method of luteoloside and acteoside were set up by HPLC.The method for fingerprint analysis using HPLC profiling of Callicarpa nudiflora was established.Ten batches samples were analyzed and 26 common peaks were selected as the fingerprint peaks of Callicarpa nudiflora.Thirteen constituents were identified by comparing their retention behaviors with standard solutions.Callicarpa nudiflora leaves and other samples were evaluated by using the chromatographic fingerprint similarity evaluation system for tradition Chinese medicine(2012).The result indicated that fingerprints of Callicarpa nudiflora leaves from diffrent batches were similar with little difference in area of common peaks and all the similarities with correlation coefficients were above 0.9.A comparative analysis showed a low similarity berween other samples and the standard fingerprint of Callicarpa nudiflora leaves.Meanwhile,little peaks were determined in Callicarpa nudiflora branches and four Callicarpa species.The established methods are highly specific and accurate,which can be used to identify Callicarpa nudiflora.The method of HPLC fingerprint was also used for simultaneous determination of caffeic acid,forsythoside B,nudifloside,luteoloside,acteoside,isoacteoside,juncein,7-O-E-p-coumaroyl-8-epi-loganic acid,luteolin,5,4’-dihydroxy-3,7,3’-trimethoxyfla-vone in Callicarpa nudiflora.Ten components showed good linearity within the test ranges.Precisions and accuracies of the method were satisfactory.The method was firstly developed for the simultaneous determination of multi-constituents for Callicarpa nudiflora in rat plasma.Three main kinds of constituents,which involved flavonoids,phenolic glycoside and terpenoids were selected as quantitative indices.An UHPLC-ESI-MS/MS method was established to determine luteoloside,dracocephaloside,juncein,acteoside,isoacteoside,forsythoside B and nudifloside in rat plasma.All analytes showed good regression(R~2>0.99)with linear ranges.The RSDs of intra-day and inter-day precisions were within 8.8%.The mean extraction recoveries of analytes were in the range 75.2%～89.9%and no significant matrix effect existed on the ionization.The developed LC-MS method was successfully applied to the pharmaeokinetie studies for seven active components in rat plasma.The results showed that seven analytes exhibited quick absorption with peak concentrations occurring at around 30 min and were eliminated rapidly thereafter.