| In this experiment,OCAP was extracted from the whole grass with hypoglycemic effect,and combined with trivalent chromium ion to prepare OCAP chromium complex.Pharmacodynamic experiments were carried out to study its hypoglycemic effect.Preparation of Ornithogalum caudatum polysaccharide chromium complexes and study its hypoglycemic activity.The main contents of this parper are as follows:(1)The extraction and purification of OCAP:using water extraction and alcohol precipitation method to extract OCAP,and refining by Sevag method and dialysate bag desalination method.(2)Study on the hypoglycemic activity of Ornithogalum caudatum polysaccharide:this experiment use the alloxan electively destroys islet beta cells in mice and gradually increases blood glucose,and then establishes a diabetic mouse model.After continuous perfusion of 14 d in mice,the effects of each dose group on weight,blood sugar,total cholesterol and triglyceride in diabetic mice were studied.The results showed that the high,medium dose group of Ornithogalum Caudatum Ait polysaccharide could relieve the symptoms of weight loss in diabetic mice.It can be seen from the data that the high dose of OCAP has a significant effect.It is suggested that OCAP have a certain therapeutic effect on the diabetic mice caused by alloxan,and the different dosage groups have a certain control effect on the total cholesterol and triglyceride in diabetic mice,and are dose-dependent.(3)Study on Synthesis and properties of OCAP complex:the refined Ornithogalum Caudatum Ait polysaccharide was dissolved in distilled water,slowly strengthen the alkaline solvent and water free three chromic chloride solution to the solution,adjust the solution PH value between eight and nine,when the solution precipitates,it is isolated and collected.Its physicochemical properties showed that OCAP chromium complex is a stable compound formed by trivalent chromium ion and OCAP.The conductance properties of the measurement results show that OCAP accounts for main role in OCAP chromium complexes in nature of the conductance.The viscosity properties results show that the viscosity of the polysaccharide chromium complex was mainly determined by the Ornithogalum polysaccharide.(4)The structural characterization of OCAP chromium complex:the polysaccharide chromium complex of Ornithogalum polysaccharide were characterized by many structral analysis methods,such as ultraviolet spectrum,infrared spectrum,fluorescence spectrum,thermogra-vimetric analysis,transmission electron microscope,particle size and particle size distribution,etc.We used UV and fluorescence spectroscopy to characterize complexes.The results show that the combination of OCAP with metal chromium ion,and chromium ion with OCAP sites on the sugar association of hydroxyl groups.The results of thermogravimetric analysis showed that when the temperature was greater than 320 degrees,the Ornithogalum caudatum polysaccharide chromium complex was stable.Transmission electron microscopy showed that the diameter of OCAP chromium complex was 40-50nm.(5)Study on the hypoglycemic activity of Ornithogalum caudatum polysaccharide:to observe the effects of OCAP and OCAP chromium complexs on the body weight,blood sugar,total cholesterol and triglyceride in diabetic mice.The data showed that the high dose group of OCAP chromium compound and OCAP can obviously relieve the symptoms of weight loss in diabetic mice.OCAP and each dose OCAP chromium complexs have certain hypoglycemic activity,and the therapeutic effect of chromium on OCAP is better than that of OCAP ligand itself.OCAP and each dose OCAP chromium complexs have certain dose dependence.OCAP and OCAP chromium complexs can reduce the level of triglyceride and total cholesterol in diabetic mice.To sum up,this paper a new type of OCAP chromium complex was synthesized by complexing with trivalent chromium ion and natural OCAP as ligands.Compared the hypoglycemic activity of OCAP and OCAP polysaccharide chromium complex.It is of great significance to development of new organic chromium assisted hypoglycemic food and the hypoglycemic drugs of Ornithogalum. |
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