Effect Of Oral Administration Of Different Probiotics On Cellular Immunity In Premature Infants

Objective:To study the effect of different probiotics on the immune status of premature infants,and to provide more scientific basis for the rational selection of probiotics in premature infants.Methods:We selected 80 preterm infants with gestational age of 340/7～366/7 weeks who were born from October 2018 to March 2020 in the Department of Obstetrics in our hospital and admitted to the intensive-care unit.According to the random number table,the patients were divided into three groups:Changlekang Group,Peifeikang Group,Mamiai Group and Control Group,20 cases in each group.Finally completed 80 cases,the control group completed 20 cases,20 cases of all experiments were completed.The preterm infants in the control group were given artificial feeding,and the preterm infants in the experimental group were given different probiotics.The premature infants in the Changlekang group were given clostridium butyricum capsule 0.210 times 2 times per day while the premature infants in the Peifeikang Group were given 0.105 times 2 times per day while the premature infants in the Changlekang group were given Bifidobacterium capsule The premature infants in the Mamiai group were given orally 0.5 G/day,2 times/Day when the milk was opened and the viable particles of Bacillus subtilis were added.The T lymphocyte subsets were measured before and after 7 days of continuous treatment with probiotics at the beginning of feeding.General basic information was collected,including Gestational Age,sex,weight,mode of delivery,time of opening milk,whether bovine pulmonary surfactant(PS)was applied after birth,and whether jaundice existed.All preterm infants were tested for hematologic and fecal abnormalities,lymphocyte,and biochemical markers before treatment.Results:The Control Group,Changlekang Group,Peifeikang Group and Mamiai group were 20 cases in each group,there was no significant difference between the control group and the experimental groups in Gestational Age,sex,body weight,mode of delivery,time of opening milk,whether to use bovine pulmonary surfactant(PS)after birth and whether there was jaundice(p>0.05).After 7 days of treatment,there was no statistical difference in the ratio of CD3+between the control group and the experimental group(after treatment/before treatment)(p>0.05),and there was no statistical difference in the ratio of CD8+(after treatment/before treatment)(p>0.05)The CD4+ ratio(after treatment/before treatment)between the Control Group and the Changlekang group was statistically different(p<0.05),and the CD4+ratio(after treatment/before treatment)between the Control Group and the Peifeikang group was statistically different(p<0.05),the CD4+ratio(after/before treatment)between the control group and the Mamie Ai Group had no statistical difference after treatment(p>0.05)The ratio of CD4+/CD8+ between control group and Changlekang Group(after treatment/before treatment)was statistically different(p<0.05),the ratio of CD4+/CD8+ between control group and Peifeikang Group(after treatment/before treatment)was statistically different(p<0.05),the ratio of CD4+/CD8+ between control group and Mamiai Group(after/before treatment)had no statistical difference after treatment(p>0.05)There was no significant difference in the ratio of t lymphocyte before and after treatment between the two groups(p>0.05),and there was no significant difference in CD4+between the two groups before and after treatment(p>0.05),there were statistical differences in CD4+between the two groups after treatment(p<0.05),between the two groups after treatment(p<0.05),between the two groups after treatment(p<0.05)and between the two groups after intervention(p<0.05)There was no statistical difference in CD4+/CD8+ between the control group before and after intervention(p>0.05),and CD4+/CD8+ between the Changlekang group and the control group after treatment(p<0.05)There were statistical differences in CD4+/CD8+between the two groups(p<0.05),and there was no statistical difference in CD4+/CD8+between the two groups(p>0.05).There were no cases of feeding intolerance such as necrotizing enterocolitis,Septicemia,frequent vomiting and abdominal distension in the preterm infants during the study period.Conclusion:1.The T lymphocyte(CD4+,CD4+/CD8+)of premature infants treated with clostridium butyricum and bifidobacterium triplex were higher than those in the control group.2.Clostridium butyricum and clostridium Bifidobacterium can improve the cellular immune function of premature infants,but there is no significant difference between them.3.Bacillus subtilis has no obvious effect on improving the function of immune cells in premature infants.