Comparative Analysis Of The Correlation Between Blood Inflammatory Indexes And Cardiac Function In Male And Female Patients With Acute Myocardial Infarction

Objective:Systematic analysis of the correlation between peripheral blood inflammation indicators and cardiac function in male and female patients with acute myocardial infarction(AMI)in Yanbian area,and compared with different types of patients,in order to correctly judge the preoperative diagnosis and postoperative treatment of patients with acute myocardial infarction and provide theoretical basis for patient maintenance.Methods:1.The first incidence of AMI who were admitted to the cardiology ward of our hospital from 2016 to 2018 and received emergency Percutaneous Coronary Intervention(PCI)were collected for analysis(343 patients).A total of 343 patients from 2016 to 2018 were selected to compare the differences in inflammatory cells between 214 male patients(Man)and 129 female patients(Women).2.Pearson’s correlation was used to statistically analyze the relationship between NLR and CK,CK-MB or EF and FS.These results were compared with the results of the previous 715 patients with acute myocardial infarction left and right coronary lesions.3.Cardiomyocytes(H9C2)were cultured,IL-6 was treated separately,and Man and Women venous sera were used to detect the expression of iNOS protein by immunoblotting.The effects of inflammatory factors and serum treatment on protein expression were observed.4.Collect the blood sample of 13 Man and 12 Women patients respectively,take serum samples for Human Cytokine Anti body Array experiment to measure and compare the differences of inflammatory factorsResults:1.In the blood examination,the hemoglobin,neutrophil count and monocyte count and CK value of Man patients were significantly higher than that of Women patients;NLR showed an increasing trend.Women patients had significantly higher platelet counts than Man.The indexes of FS%,CO,SV and E/A were significantly higher than those of Women in the ultrasonic cardiac function test indexes.2.The ratio of neutrophil to lymphocyte ratio(NLR)is an important prognostic indicator for patients with acute myocardial infarction.Correlation analysis showed that In Man patients,NLR was negatively correlated with EF%,FS%,SV,and CO(respectively:r=-0.243,P<0.0001;r=-0.238,P<0.0001;r=-0.163,P=0.017;r=-0.189,P=0.006).In Women patients,NLR was weakly correlated with EF%and FS%(r=-0.177,P=0.044;r=-0.171,P=0.053).Unlike male patients,E/A was correlated with NLR performance in Women patients(r=0.18,P=0.041).3.In this group of patients,CK,CK-MB and NLR showed no correlation between Man and Women myocardial injury indicators(Man:r=-0.001,P=0.986,r=-0.011,P=0-898;Women:r=0.004,P=0.959,r=-0.006,P=0.942).4.Cytokine array assay in venous serum of Man and Women patients revealed that the expression of IL-1ra,IL-2,IL-11,IL-17 and MCSF in Man group were increased(P<0.05).5.Western blotting experiments revealed that iNOS expression in H9C2 cells are increased with the venous sera of the Man and Women patients treatment.But there was no difference between the Man and Women groups(P>0.05).6.NLR was negatively correlated with EF and FS in 715 patients with acute myocardial infarction.However,when divided into left and right coronary lesions,NLR did not show correlations with functional parameters of the heart.NLR and CK,CK-MB showed significant correlations,especially in patients with left coronary lesions.Conclusion:1.The inflammatory index(neutrophils and monocytes)in the peripheral blood of male patients with acute myocardial infarction is significantly increased,and the degree of myocardial injury is more serious.NLR has an increasing trend in male patients and is negatively correlated with cardiac function indexes.NLR is an informative inflammation index to predict the severity in male patients with acute myocardial infarction,2.NLR and acute myocardial injury are positively correlated in patients with acute myocardial infarction.However,NLR and myocardial injury did not show correlation after re-grouping(e.g.male and female,left and right coronary artery infarction).The relationship between NLR and myocardial injury needs further analysis.3.Distinctive serum inflammation indexes are observed in male and female patients.