| Psoriasis is a chronic,inflammatory skin disease with a long course and easy recurrence.Some patients can hardly be cured for a lifetime,which seriously affects the work and life of the patients.Therefore,it is of great significance to explore effective methods for the treatment of psoriasis.Studies have shown that oxidative stress and inflammation play important roles in the pathogenesis of psoriasis.Selenium(Se)has many biological functions such as antioxidant and immune regulation.There is a large body of evidence that the Se level in psoriasis patients is lower than that of healthy individuals,however,the underlying mechanism is not clear.Vitamin E(VE)and coenzyme Q10(Co Q10),as major antioxidants,play important antioxidant roles in the body.In the present work,we constructed an IMQ-induced psoriasis animal model to study the effect of Se yeast,VE,Co Q10 and combined supplementation on psoriasis in mice,as well as the related mechanism,which will provide a new clue for the treatment of psoriasis.The main results are as follows:(1)The effects of Se yeast at different doses on IMQ-induced psoriasis in mice.We applied IMQ to the exposed back skin of BALB/c mice for 7 days,the body weight decreased significantly,the epidermal thickness and PASI score increased significantly.Compared to the IMQ group,the Se content in the liver and the activity of GPx in serum were increased significantly,while the content of MDA in serum was decreased significantly after supplementation with Se yeast at different doses(25、50、100 μg Se/kg·bw).However,the epidermal thickness and PASI score did not change significantly by treatment with Se yeast.The results of histopathological staining showed that different doses of Se yeast had no obvious toxicity on the kidney,but high-Se yeast(100 μg Se/kg·bw)had side effects,as demonstrated by a certain degree of damage in the liver of mice.These results indicated that supplementation with different doses of Se yeast increased antioxidant capacity in mice,but had no improvement on the IMQ-induced psoriasis in mice.(2)Effects of the supplementation with the two of Se yeast,VE and Co Q10 on IMQ-induced psoriasis in mice.Compared with the IMQ group,the GPx activity in serum,and the activities of CAT and GPx activity in liver were significantly increased,and the MDA level in skin tissue was significantly decreased,but there were no significant changes in PASI score and the epidermal thickness after supplemented with Se yeast plus VE.Similarly,supplementation with Se yeast plus Co Q10 or supplementation with VE plus Co Q10 had no obvious improvement on the psoriatic lesions in mice.These results suggested that the supplementation with the two of Se yeast,VE and Co Q10 had not effects on the IMQ-induced psoriasis in mice under the present experimental condition.(3)Effect of supplementation with the combination of Se yeast,VE and Co Q10 on IMQ-induced psoriasis in mice.Compared with the IMQ group,combination supplementation significantly attenuated the IMQ-induced psoriasis in mice,as showed by the significant decrease of the scales,the PASI score,the indexes of liver and kidney,the thickness and area of epidermis,the proliferation of epidermal cells and the infiltration of T cells.Further studies found that the activity of GPx in serum,liver and skin,the activity of CAT in liver were significantly increased,while the content of MDA in skin was significantly decreased after combination supplementation.However,no significant changes in levels of GSH and GSSG and the activity of SOD were observed in mice after combination supplementation.Furthermore,compared with the IMQ group,the m RNA levels of IFN-γ and IL-17 F were significantly decreased,and the m RNA levels of VEGF and TNF-α were slightly decreased.These results indicated that combination supplementation with Se yeast,VE and Co Q10 could alleviated IMQ-induced psoriasis in mice by enhancing antioxidant and reducing inflammatory factors levels. |
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