Effects Of Iron On Learning And Memory Ability Of Zebrafish Adults And Development Of Bones And Nerves Of Juveniles

Iron is a necessary trace element for the human body.There are many ways to obtain it in daily life.Iron in the human body is in a dynamic balance of continuous absorption,utilization,storage,and circulation,namely iron steady state.But after this dynamic balance is broken,it may cause iron overload or iron deficiency.Iron overload can affect the development of larvae.Iron deposits have been observed in the lesions of neurodegenerative diseases such as Alzheimer’s disease（AD）.Studies in various papers also prove that ferroptosis caused by iron overload plays a role in the formation of AD.Important role.This article uses zebrafish to build an iron overload model to study the damage of iron overload to the nervous system of adult fish and the effect of iron overload on embryo development.The results are as follows:1 Build an iron overload modelBy comparing the RT-PCR results and behavioral data of 6-month-old zebrafish adults treated with different concentrations and times,an iron overload model with p H5.8 of FeCl3 solution for 4 weeks was successfully constructed.Compared with the blank control group,the time for the model group to enter the EC zone increased significantly,and the expression of various genes changed significantly.The zebrafish embryos were treated with different concentrations,and their mortality was counted,and FeCl₃LC50=7.957 mg/L(expressed as the amount of Fe³⁺)was calculated.2 The effect of iron overload on zebrafish embryo developmentZebrafish embryos of line AB were exposed to different concentrations of FeCl₃,using the Noldus system to detect their behavioral changes,statistics of their hatching rate and mortality,recording their deformity rate and deformity,RT-PCR detection of genes related to nerve and bone development Expression changes.Compared with the normal control group,various indicators of the higher-concentration iron-treated group showed significant changes,and the larger the change was with the increase of the concentration.In summary,FeCl₃ exposure may cause iron overload,and the data of related genes also indicate that iron deposition may have occurred,and the accumulated iron may damage the nervous system,resulting in the accumulation of Aβand neuroinflammation,which may lead to neuronal apoptosis.,Leading to cognitive impairment.At the same time,FeCl₃ exposure can have a significant effect on zebrafish embryo development.