Efficiencies Of P16^INK4a Immunocytochemical Staining In Screening Of HSIL+

Objective:To investigate the efficiencies of p16^INK4a immunocytochemical staining in screening of high-grade cervical intraepithelial neoplasia and cervical cancer（HSIL+）,and to propose a more positive and effective screening plan to improve the accuracy of cervical cancer screening.Methods:A total of 106 patients with abnormal results after cervical cancer screening who needed cervical biopsy under colposcopy were selected as the research objects,and p16^INK4aimmunocytochemical staining was performed in the cervical exfoliated cells.The cervical biopsy histopathological results were used as the gold standard.The correlation between the positive rate of p16^INK4a immunocytochemical staining and the level of cervical lesions was investigated.The efficiencies of p16^INK4a immunocytochemical staining in screening of HSIL+were analyzed.By comparison with TCT,the values of p16^INK4a immunocytochemical staining in triaging the HPV 16/18 positive women and the women positive for 12 other HPV genotypes were evaluated in this study.Results:The positive rate of p16^INK4a immunocytochemical staining increased with the level of cervical lesions（χ²=50.143,P<0.001）.The sensitivity,specificity,positive predictive value,negative predictive value and diagnostic accuracy of p16^INK4a immunocytochemical staining for HSIL+were 94.12%,79.17%,68.09%,96.61%,83.96%,respectively.The sensitivity（χ²=3.693,P=0.055）and specificity（χ²=1.418,P=0.234）of p16^INK4a immunocytochemical staining were similar to those of TCT in detection of HSIL+in the HPV 16/18 positive women.The sensitivity（P=1.000）of p16^INK4a immunocytochemical staining was similar to that of TCT in detection of HSIL+in the other 12 types of HPV positive women,while the specificity（χ²=25.379,P<0.001）was higher than that of TCT.Conclusion:（1）The positive rate of p16^INK4a immunocytochemical staining is closely related to the level of cervical lesions.（2）p16^INK4a immunocytochemical staining has high screening efficiency for HSIL+.（3）Compared with TCT,the combination of HPV testing and p16^INK4a immunocyto-chemical staining is helpful to improve the accuracy of cervical cancer screening.