Clinical Study On The Effect Of ’Enriching Yin And Removing Toxin Prescription’ Combined With Chemotherapy On The Expression Of β3GnT8 And CD147 In Serum Of NSCLC Patients

Objective:Under theoretical guidance of ’Carcinogenesis of Du’,this research aims to analyze the expression of β3GnT8 and CD 147 in serum among patients with non-small cell lung cancer(NSCLC)and discuss the connection between these two indexes and metastasis.The function of the two groups classified by whether using traditional Chinese medicine ’Enriching Yin and removing toxin prescription’ or not will be observed,by focusing on the following indexes:the expression of β3GnT8,CD147,CEA,CA125 in serum,the number of immune cells,the TCM syndrome efficacy score and weight change.As the results get,the mechanism of the prescription will be discussed.Methods:From 2019.10 to 2021.01,we collected 90 NSCLC patients and 40 healthy volunteers from three hospitals in Suzhou to record and analyze the relationship between generally condition and the expression of β3GnT8 and CD 147 in serum.The differences of two indexes in patients with or without metastasis were researched as well.After that,we listed 50 cases of them which satisfied with the criteria for inclusion and exclusion as experimental research objects,and classified them in two groups:observation group and control group.During the observation process,3 cases in the observation group and 12 cases in the control group could not complete the study on time due to missing data and any other serious adverse events.Factors of these 15 cases were eliminated.The whole course of observation was finally completed in 35 cases,including 22 cases in the observation group and 13 cases in the control group.The control group was given a chemotherapy regimen according to the NCCN clinical practice guidelines for NSCLC lung cancer(2019.7version),and the observation group was treated with ’Enriching Yin and removing toxin prescription’ and chemotherapy at the same time.All patients took 3 weeks as a course of treatment,and two courses were administered.After 2 courses of observation,the indexes mentioned above and other related safety indicators were analyzed with statistical software and conclusions were discussed.Results:The expression levels of β3GnT8 and CD147 in serum are different between healthy people and patients suffering from NSCLC.The expression level of NSLCL patients is significantly higher than that of healthy people(P<0.01),and are significantly higher in those patients with metastasis(P<0.05).In the research of Yin Deficiency Toxic Heat Syndrome,compared with the control group,the observation group showed therapeutic advantages in all aspects,and both groups can reduce the β3GnT8 and CD 147 expression in serum,and the effect in the observation group was significantly better than that of the control group(P<0.01).However,there was no difference in the numerical changes in the tumor markers CEA and CA125(P>0.05).In terms of immune cells after treatment,the immune function of the control group decreased,with the most obvious changes in CD4+count and CD4+/CD8+ ratio(P<0.01).The immune function indexes of the observation group changed significantly(P<0.01)and the immune function improved.After treatment,the scores of the observation group significantly reduced than before(P<0.01)in TCM syndrome,while the changes in the control group were not(P>0.05).From the perspective of weight change,the statistical results of both within-group and between-groups comparisons are meaningless(P>0.05).In terms of safety indicators,except that the white blood cell and platelet count in the control group decreased significantly after treatment(P<0.01)and the ALT in the observation group increasd after treatment(P<0.05),there was no significant difference in other safety indicators before and after treatment(P>0.05).The overall adverse reaction rate in the observation group was lower than that in the control group,which shows that TCM can reduce the side effects of chemotherapy.To conclude,TCM has the effect of reducing toxicity and increasing efficacy.Conclusion:①The expressions of β3GnT8 and CD147 in serum of patients with NSCLC are higher than that of healthy people,especially in those with metastasis;②The combined use of’Enriching Yin and removing toxin prescription’ and chemotherapy can reduce the expression ofβ3GnT8 and CD147 in serum,and improve the TCM syndrome.The degree of change is more significant than that of chemotherapy alone;③Comparing with chemotherapy alone,combined treatment of TCM and chemotherapy has positive effects on improving immunity of patients and treatment safety.