Analysis Of IL-25 And IL-33 Expression In Induced Sputum In Patients With Chronic Obstructive Pulmonary Disease

ObjectiveTo study the concentration of IL-25 and IL-33 in induced sputum of patie nts with chronic obstructive pulmonary disease in acute phrase to preliminarily clarify the correlation between the classification of the diseases,explore differ ent types of airway inflammation and the correlation between the two inflamm atory factors,which provides a reference for exploring the mechanism of airwa y inflammation in chronic obstructive pulmonary disease.MethodFrom October 1,2019 to October 1,2020,patients with chronic obstructive pulmonary disease were recruited in the outpatient and inpatient department of the respiratory medicine department of the Third Affiliated Hospital of Southern Medical University.All patients were collected for detailed basic information,referring to the 2018 GOLD guidelines and the diagnostic criteria,and divided into four groups,including 4 cases of grade Ⅰ,4 cases of grade Ⅱ,11 cases of grade Ⅲ,12 cases of grade Ⅳ,22 males and 9 females.From October 1,2019 to October 1,2020,11 patients with chronic cough were recruited in the outpatient department of respiratory medicine of the Third Affiliated Hospital of Southern Medical University,including 6 males and 5 females.Both groups of patients were tested including lung function,induced sputum and concentrations of IL-25,IL-33 in sputum supernatant.Result1.Comparing from the IL-25 concentration level within COPD group,co mpared with grade Ⅰ,the difference of grade Ⅳ is significant(P<0.01).Comp ared with grade Ⅱ,the difference of grade Ⅲand Ⅳ is significant(P<0.01).T here was no significant difference of the IL-25 concentration level between tw o groups(P>0.05).2.Comparison of IL-33 concentration levels between the chronic cough gr oup and the COPD group:In the COPD group,compared with grade Ⅰ,the di fference of IL-33 concentration of grade Ⅲand grade Ⅳ was significant(P<0.05).Compared with grade Ⅱ,the difference in IL-33 concentration level betwee n grade Ⅲ and grade Ⅳ was significant(P<0.01).In comparison between two groups,the IL-33 concentration of grade Ⅲ and Ⅳ in induced sputum was si gnificantly different(P<0.05).3.The concentration of IL-25 is positively correlated with the concentratio n of IL-33 in the COPD group(r=0.617,P<0.01).4.Results of induced sputum in the COPD group:5 cases of neutrophil p henotype in chronic cough group,6 cases of paucigranulocytic phenotype;5 ca ses of neutrophil phenotype in COPD group(2 cases of grade Ⅰ,1 cases of grade Ⅱ,0 cases of grade Ⅲ,2 cases of grade Ⅳ),11 cases of eosinophil ty pe(1 cases of grade Ⅰ,2 cases of grade Ⅱ,6 cases of grade Ⅲ,2 cases of grade Ⅳ),13 cases of mixed granulocyte type(1 case of grade Ⅰ),1 case of grade Ⅱ,4 cases of grade Ⅲ,7 cases of grade Ⅳ),2 cases of oligocellu lar type(0 case of grade Ⅰ,0 case of grade Ⅱ,1 case of grade Ⅲ,1 case of grade Ⅳ).Conclusion1.The airway phenotypes in the COPD group are mainly eosinophilic and mixed granulocytic phenotype,and the airway phenotypes in the chronic coug h is mainly neutrophilic and paucigranulocytic phenotype.2.In patients with chronic obstructive pulmonary disease,the concentration of IL-25 and IL-33 are different in different disease severity levels.Patients with severe COPD have higher concentrations of IL-25 and IL-33 than patients with mild to moderate COPD,suggesting that IL-25 and IL-33 may be used as a predictor of severity.3.Both IL-25 and IL-33 are significantly related to airway obstruction.Th e more severe of the airway obstruction,the higher of the concentration level,which may be used as a potential predictor and potential targets of the future research progression of chronic obstructive pulmonary disease.