Effect Of Ganningfang On Intestinal Flora Of Patients With Chronic Hepatitis B

Objective : To observe the clinical efficacy of Ganningfang,an experienced prescription of National famous old Chinese medicine Professor Lan Qingqiang,on patients with chronic hepatitis B(CHB),and to analyze and evaluate the influence of Ganningfang on intestinal flora of patients with chronic hepatitis B.Methods:In this study,64 patients who were diagnosed as CHB in the Department of Gastroenterology and Hepatology of Ruikang Hospital affiliated to Guangxi University of traditional Chinese Medicine from March2020 to December 2020 were randomly divided into observation group(n =32)and control group(n = 32).The control group was treated with entecavir dispersible tablets and the observation group was treated with entecavir dispersible tablets plus Ganningfang.Six months after treatment,the changes of TCM clinical symptom score,liver function "ALT,AST,TBIL",hepatitis B virus nucleic acid(HBV-DNA)quantification,HBe Ag serological negative conversion rate,serum inflammatory factors "IL-6,IL-12" and intestinal flora "Escherichia coli,Enterococci,Lactobacillus,Bifidobacterium" were observed in the two groups before and after treatment.Results:(1)Overall efficacy comparison: the overall effective rate of the control group was 73.33%,while that of the observation group was 93.33%.Overall,the overall effective rate of the observation group was significantly higher than that of the control group,and the difference was statistically significant(P<0.05).(2)Comparison of TCM clinical symptom scores: compared with those before treatment,the total scores of TCM clinical symptoms in the two groups after treatment were significantly lower than those before treatment,and the difference was statistically significant(P<0.05).Comparison between the two groups after treatment,the total score of TCM clinical symptoms in the observation group was significantly lower than that in the control group,and the difference was statistically significant(P<0.05).(3)Comparison of liver function indexes: compared with those before treatment,the serum levels of "ALT,AST,TBIL" in the two groups decreased significantly after treatment,and the difference was statistically significant(P<0.05).Comparison between the two groups after treatment,the serum levels of "ALT,AST,TBIL" in the observation group were significantly lower than those in the control group,and the difference was statistically significant(P<0.05).(4)Comparison of HBV-DNA quantitative level:compared with those before treatment,the quantitative level of HBV-DNA in the two groups decreased significantly after treatment,and the difference was statistically significant(P<0.05).Comparison between the two groups after treatment,the difference was not statistically significant(P>0.05).(5)Comparison of HBe Ag serological negative conversion rate :Comparison between the two groups after treatment,there was no significant difference in HBe Ag serological negative conversion rate,the difference was not statistically significant(P>0.05).(6)Comparison of the levels of serum inflammatory factors: compared with those before treatment,the levels of serum inflammatory factors "IL-6,IL-12" in the two groups after treatment were significantly lower than those before treatment,and the difference was statistically significant(P<0.05).Comparison between the two groups after treatment,the levels of serum inflammatory factors "IL-6,IL-12" in the observation group were significantly lower than those in the control group,and the difference was statistically significant(P<0.05).(7)Comparison of the level of intestinal flora: the level of intestinal flora "Lactobacillus,Bifidobacterium,Escherichia coli and Enterococcus" in the control group was not significantly different from that before treatment,and the difference was not statistically significant(P>0.05).In the observation group,after treatment,the levels of intestinal probiotics "Lactobacillus and Bifidobacterium" were significantly higher than those before treatment,while the levels of intestinal pathogenic bacteria "Escherichia coli and Enterococcus" were significantly lower than those before treatment,and the difference was statistically significant(P<0.05).After treatment,the levels of intestinal probiotics "Lactobacillus and Bifidobacterium" in the observation group were significantly higher than those in the control group,while the levels of intestinal pathogenic bacteria "Escherichia coli and Enterococci" in the observation group were significantly lower than those in the control group,and the difference was statistically significant(P<0.05).Conclusion:(1)Ganningfang combined with entecavir has obvious curative effect on patients with CHB,which can improve clinical symptoms,reduce viral replication level,improve liver function,and regulate intestinal microflora by inflammatory response.(2)Both treatment methods can improve the clinical symptoms of CHB patients,liver function inflammation,injury and virus replication level,but the Ganningfang group has more obvious advantages than the pure western medicine group after comprehensive analysis and is significantly better than the pure western medicine group in improving intestinal flora.