Analysis Of The Relationship Between The Factors Affecting Intestinal Flora And Allergic Diseases In Children

Objective: To explore whether the intestinal microflora changes and the possible factors influencing the change of intestinal microflora in hospitalized children after antibiotic treatment;to analyze the disease characteristics of allergic children,to compare the exposure of the factors related to the intestinal microflora in infants of allergic and non allergic children,to analyze the risk factors of allergic diseases in children,to explore the occurrence of allergic diseases and The correlation of the factors affecting the intestinal flora in infancy.Methods:1.Select the children who are hospitalized in the general internal medicine ward of Tianjin Children’s Hospital from October 1,2019 to January 1,2020 and need to be treated with antibiotics.According to the inclusion and exclusion criteria,a total of 50 cases of medical records are included.Record the age,gender,pre admission and in-hospital medication and other basic information of all subjects.All subjects stay on the day of admission and three days after the node Fresh feces were collected and placed in the stool retention box.The staff immediately sent them to the microorganism room for detection.The overall situation of intestinal flora and the number and proportion of various bacteria before and after the application of antibiotics were compared to explore whether the intestinal flora changed after the application of antibiotics in children in hospital and the possible factors affecting the degree of intestinal flora change.2.From June 1,2019 to December 1,2019,367 children with allergic diseases(including eczema,urticaria,food allergy,asthma,allergic cough,allergic rhinitis,allergic conjunctivitis and drug allergy)who were hospitalized in the second respiratory ward of Tianjin Children’s hospital were selected.According to the inclusion and exclusion criteria,367 cases were included in the medical records.Meanwhile,the same period of hospitalization was selected 185 children who met the corresponding conditions but had no allergic diseases were taken as the control group.The general information of all the subjects,the possible factors affecting the intestinal flora in infancy and the prevalence of allergic diseases were recorded.The percentageand absolute value of eosinophils and basophils in the last blood routine as well as the serum Ig E level were registered during the hospitalization,and the information was missing Use face-to-face inquiry or telephone follow-up.To compare the exposure of two groups of children to the related factors of intestinal flora in infancy,and analyze the risk factors of allergic diseases in children.Results:1.The results of Gram staining showed that the proportion of Gram-positive bacteria decreased significantly(P < 0.05);the proportion of Gram-positive bacteria increased significantly(P < 0.05);the average number of bacteria per field,the proportion of Gram-negative bacteria and Gram-negative bacteria had no significant difference(P >0.05).According to the age,gender and antibiotic usage of children,there was no significant difference in the distribution of fecal bacteria in each group(P > 0.05).2.The incidence of atopic dermatitis,allergic rhinitis and urticaria were 77.93%,45.78% and 30.52% respectively in the allergic group.The prevalence of asthma,food allergy,allergic cough,drug allergy and allergic conjunctivitis were 19.89%,18.80%,16.35%,11.44% and 7.63% respectively.104 cases(28.34%)had only one allergic disease,263 cases(71.66%)had two or more allergic diseases.Among the allergic children over 7 years old,the highest incidence of atopic dermatitis is 0-1years old,food allergy is most common around 1 year old,and the incidence of respiratory allergy increases with age.The proportion of children with allergic disease family history in allergic group was higher than that in non allergic group(P < 0.05).The serum Ig E level in allergic group was significantly higher than that in non allergic group(P < 0.05).There was no significant difference in sex,age,BMI,percentage and absolute value of eosinophils and basophils between the two groups(P > 0.05).The proportion of urban residents in allergic group was higher than that in non allergic group(P < 0.05).The proportion of the children with exclusive breastfeeding time less than 6 months in allergic group was higher than that in non allergic group(P < 0.05).There was a significant difference between allergic group and non allergic group(P < 0.05).The proportion of children in allergic group who had taken probiotics orally was significantly higher than that in non allergic group(P< 0.05).There was no significant difference between the two groups in the only child,the mode of production and the time of supplement(P > 0.05).Conclusion: 1.After antibiotic treatment,the intestinal flora of hospitalized children changed,mainly manifested in the decrease of Gram-positive bacteria and the increase of gram-positive cocci.2.In allergic children,the incidence of eczema,allergic rhinitis and urticaria is higher than that of other allergic diseases.Most allergic children have two or more kinds of allergic diseases.Children’s allergies progress from skin and digestive tract allergies to respiratory tract allergies.3.Exposure to antibiotics within one year after birth is a risk factor for allergic diseases in children.4.Children living in cities and towns are more likely to have allergic diseases.5.Pure breastfeeding to the age of 6 months after birth is beneficial to reduce the incidence of allergic diseases in children.