| Purpose: The tripartite motif-containing(TRIM)protein family has been proved to abnormally expressed and prognostic value in certain tumors.However,it is not clear whether TRIM family is highly expressed or underexpressed in melanoma,and whether it affects patient prognosis.The objective of this article was to distinguish that whether TRIM protein is differentially expressed in melanoma and has an impact on survival.Methods: In this paper,we used Oncomine database and GEPIA database to compare the transcriptional levels of TRIM family genes in cancer tissues and normal samples,and screened out the members of TRIM family with differential expression.In order to elucidate the function,biological process and enrichment pathway of the selected genes,annotation,visualization and integrated discovery database(DAVID database)was used to perform GO and KEGG enrichment analysis.The protein expression of selected genes in melanoma and normal skin tissue was explored in the human protein map database(HPA database),and the protein expression of Trim family in melanoma was screened out.The correlation analysis was carried out in three aspects.First,we conducted a correlation analysis of selected key genes.Subsequently,the UCSC database was used to search and obtain clinicopathological data and gene expression profiles of patients,and the relationship between TRIMs and clinical parameters of melanoma patients was determined.We also used the Tumor Immune Assessment Resource(TIMER)platform to investigate the relationship between tumor immunoinfiltrating cells(TIICs)and TRIMs on the basis of overall survival(OS)and disease-free survival(DFS).The prognostic significance of TRIM2,TRIM7,TRIM8,TRIM18(MID1),TRIM19(PML),TRIM27 and TRIM29 in melanoma was evaluated by GEPIA.At the same time,polymerase chain reaction(q RT-PCR)was used to verify the real-time quantification of TRIM family proteins after screening.Finally,a gene-set enrichment analysis(GSEA)of TRIM27 was performed using the Linkedomics database to explore its biological function and its associated inhibition or activation signaling pathways.Results:By screening differential genes in TRIM family,seven genes including TRIM2,TRIM7,TRIM8,TRIM18(MID1),TRIM19(PML),TRIM27 and TRIM29 were found to be differentially expressed in melanoma and normal tissues.According to the observation,there are different protein expression patterns of TRIMS in melanoma.Compared with melanoma,TRIM7 and TRIM29 can be detected and highly expressed in normal skin tissue cells,while TRIM27 is weakly expressed in skin tissue and strongly positive expressed in melanoma sections.Tumor type and stage were used as grouping indexes to observe the gene expression of TRIM family members,and it was found that TRIM19 was highly expressed in most tumor types and stages,while TRIM7 and TRIM29 were mostly low expressed in metastatic melanoma,indicating that TRIM7 and TRIM29 were helpful in differentiating primary and metastatic tumors.Prognostic analysis of TRIM family in melanoma was studied,and the results showed that the high expression of TRIM18,TRIM27 and TRIM29 was significantly correlated with the survival rate of patients,and only TRIM27 was significantly correlated with the disease-free survival rate of melanoma.The expression analysis of TRIMS gene in melanoma cell lines by q RT-PCR further confirmed that the prognosis was significant.The results showed that compared with the normal control,the expression of TRIM27 was high in the melanoma cell line A375,and the expression of TRIM29 was low in the melanoma cell line A375,both of which were statistically significant.The results of TRIM27 gene enrichment showed that biological regulation and metabolism could not be separated from TRIM27,which also plays an important role in membrane,nucleus,protein binding and ion binding,and also plays a certain auxiliary role in ribosome and cytokine-cytokine receptor interactions.Conclusions: This study discovered that TRIM27 may be considered a new potential biomarker for melanoma. |
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