Changes Of T Lymphocyte Subsets And Neutrophil-Lymphocyte Ratio In Patients With Cervical Cancer Before And After Radiotherapy

Objective:By analyzing the changes of T lymphocyte subsets and neutrophil to lymphocyte ratio(NLR)in cervical cancer patients,to understand the immune status of cervical cancer patients before and after radiotherapy,and to explore the influence of radiotherapy on the immune status of cervical cancer patients,so as to provide reference for cervical cancer patients with radiotherapy combined with immunotherapy.Methods:A total of 61 patients with cervical cancer undergoing radiotherapy in the First Central Hospital of Baoding City from January 2019 to December 2020were selected as the research subjects,aged 29 to 76 years old,with a median age of 60 years old,including 32 patients with age≤60 years old and 29patients with age>60 years old.Under the 2018 international association of gynecology and obstetrics(FIGO)staging patients divided into two groups,36patients withⅠ-Ⅱperiod,25 patients withⅢ-Ⅳperiod.The changes of T lymphocyte subsets and NLR in cervical cancer patients before and after radiotherapy were analyzed statistically.The correlation between T lymphocyte subsets,NLR and age and stage of cervical cancer patients was further analyzed.P<0.05 was considered statistically significant.Results:1.Correlation analysis of T lymphocyte subsets and patients with cervical cancer1.1 The relationship between T lymphocyte subsets and age and stage in patients with cervical cancer(1)There were no significant differences in CD3~+T cells,CD4~+T cells,CD8~+T cells,CD4~+T/CD8~+T cells and Treg cells between cervical cancer patients aged≤60 years and cervical cancer patients aged>60 years(P>0.05).(2)Ⅲ-Ⅳstage cervical cancer patients with CD3~+T cells,CD4~+T cells compared with theⅠ-Ⅱperiod patients is reduced,the difference was statistically significant(P<0.05).There were no significant differences in CD8~+T cells,CD4~+T cells/CD8~+T cells and Treg cells between the two groups(P>0.05).1.2 Changes of T lymphocyte subsets in patients with cervical cancer before and after radiotherapy(1)The ratio of CD3~+T cells,CD4~+T cells and CD4~+T cells/CD8~+T cells after radiotherapy were significantly lower than that before radiotherapy,CD8~+T cells increased after radiotherapy,with statistical significance(P<0.05).(2)Treg cells increased after radiotherapy compared with before radiotherapy,and the difference was statistically significant(P<0.05).2.Correlation analysis of NLR and cervical cancer patients2.1 Relationship between NLR and age and stage of cervical cancer patients(1)There were no significant differences in neutrophils,lymphocytes and NLR between cervical cancer patients aged≤60 years and cervical cancer patients aged>60 years(P>0.05).(2)Ⅲ-Ⅳperiod neutrophils and lymphocytes in the peripheral blood of patients with cervical cancer thanⅠ-Ⅱperiod in patients with no significant difference(P>0.05),howeverⅢ-ⅣNLR thanⅠ-Ⅱperiod increased obviously,and the difference was statistically significant(P<0.05).2.2 Changes of NLR before and after radiotherapyAfter radiotherapy,neutrophils and lymphocytes decreased significantly compared with before radiotherapy,with statistical significance(P<0.05).NLR was increased after radiotherapy,and the difference was statistically significant(P<0.05).Conclusion:T lymphocyte subsets and NLR were not correlated with the age of cervical cancer patients,but were correlated with the stage,suggesting that cervical cancer patients with late stage had low immune function and obvious systemic inflammatory response.Radiotherapy can affect the immune function and systemic inflammation in patients with cervical cancer.Therefore,T lymphocyte subsets and NLR can be monitored before and after radiotherapy to evaluate the immune status of patients,so as to provide a basis for immunoregulatory therapy in patients with cervical cancer.Timely administration of immunomodulatory therapy has a positive effect on the recovery of the body’s immune function.