Correlation Between Brain Glioma And Intestinal Flora

Objective The purpose of this study was to investigate the diversity and composition of intestinal microflora between healthy people and patients with gliomas or patients with different grades of gliomas.To compare the differences of intestinal flora between healthy people and patients with glioma and patients with different grades of glioma,so as to provide potential ideas for discovering the pathogenesis of glioma and looking for individualized treatment.Methods From August 2019 to October 2020,patients with brain glioma and healthy adults who were admitted to the Department of Neurosurgery of Ningxia Medical University General Hospital and who met the criteria of physical examination were selected as the control group.There were 11 cases in healthy control group,low-grade glioma group and high-grade glioma group.The subjects were matched and analyzed according to sex,age,weight and height.Fecal DNA,was extracted from each subject’s fecal samples to amplify bacterial 16 SrDNAV3-V4 fragments,and the species structure diversity and abundance of intestinal microflora in healthy people and glioma patients were analyzed by high-throughput sequencing.Results The results of 16 SrDNA high-throughput sequencing showed that there were significant differences in the abundance of intestinal microflora between healthy people and patients with gliomas and patients with ifferent grades of gliomas at the level of gate,class,order,family and genus.In the high-grade glioma group.Euryarchaeota,Proteobacteria,Lentisphaerae,Methanobacteria,Deltaproteobacteria,Lentisphae ria,Desulfovibrionales,Enterobacteriales,Enterobacteriaceae,Clostridiales＿vadin BB60＿group,The relative abundance of Escherichia-Shigella was significantly higher than that of healthy controls.The relative abundance of Prevotellaceae＿UCG-001,Lachnospira,Ruminococcus＿gauvreauii＿group and Fusicatenibacter was lower than that of healthy controls.In the low grade glioma group,the relative abundance of Euryarchaeota,Proteobacteria,Methanobacteria,Deltaproteobacteria,Desulfovibrionales,Entero-bacteriales and Enterobacteriaceae was significantly higher than that of the healthy control group,while the relative abundance of Prevotellaceae＿UCG-001 and Roseburia was lower than that of the healthy control group.In the high-grade glioma group,the relative abundance of Lentisphaerae and Lentisphaeria in the high-grade control group was significantly higher than that in the low-grade glioma group,while the relative abundance of Ruminococcaceae＿UCG-009 in the high-grade control group was lower than that in the low-grade glioma patients.The difference was statistically significant(P < 0.05).Conclusion There are significant differences in the diversity and structure of intestinal flora between glioma patients and healthy people,such as imbalance between inflammatory flora and anti-inflammatory flora,increase of opportunistic pathogens and decrease of probiotics in glioma patients.there are also differences in the structure of intestinal microflora in patients with different grades of gliomas and have their own characteristics.these flora may be potential biomarkers of pathogenic factors of glioma.It may provide a potential target for individualized treatment of gliomas.