Study On The Nano-mechanical Properties Of Tumor Cells Based On Real-time Force Spectroscopy

The physiological state of cells is closely related to the health level of the organism.When a disease occurs in the organism,the physiological state of cell will change accordingly.The mechanical properties of cells are an important indicator of their physiological state.With the improvement of science and technology,the detection methods of cell mechanical properties are also changing rapidly.Atomic force microscope(AFM)has gradually become an important means for testing the mechanical properties of cells due to its nano-scale spatial resolution,pico Newton level force sensitivity,and ease of sample preparation.In this thesis,the morphology and mechanical properties of two tumor cells(pancreatic cancer,breast cancer)were measured using real-time force spectroscopy and the changes in the mechanical properties of tumor cells after treatment with anticancer drugs were also studied.The main research contents include the following parts:Firstly,structure and mechanical properties between normal pancreatic cells and three pancreatic cancer cells were compared.The results show that the Young’s modulus of normal cells is greater than that of corresponding cancer cells.And the Young’s modulus of different pancreatic cancer cells is different.The Young’s modulus of the more aggressive cancer cell As PC-1 is smaller than that of the less aggressive cancer cell Bx PC-3.Secondly,the structure and mechanical properties of normal breast cells and three breast cancer cells were studied.The order of the Young’s modulus values of the four cells is listed as following: normal breast epithelial cells HBL-100(10.72 ± 2.72KPa)> human breast adenocarcinoma cells SK-BR-3(5.81 ± 1.73 KPa)> breast cancer cells MDA-MB-231(4.85 ± 1.81 KPa)> human breast cancer cell MCF-7(2.75 ± 1.12 KPa),and the distribution range of Young’s modulus of normal cells is wider than that of cancer cells.Finally,the effects of two anticancer drugs on mechanical properties of two type tumor cells were studied.The results show that the Young’s modulus of both types of cancer cells changed after treatment with anticancer drugs,and different drugs have different effects on the Young’s modulus of cancer cells.After treatment of cancer cells with adriamycin hydrochloride,the Young’s modulus of breast cancer cells MDA-MB-231 and pancreatic cancer cells MIA Pa Ca-2 show an increasing trend.The more concentration of adriamycin hydrochloride,the higher the Young’s modulus of cancer cells.After emodin treatment of cancer cells,the Young’s modulus of breast cancer cells MDA-MB-231 showed a downward trend,but the effect of emodin on the Young’s modulus of cancer cells requires a certain period of time.Studying the difference between the mechanical properties of cancer cells and normal cells can provide new ideas for the early diagnosis of cancer.In addition,studying the mechanical properties of cancer cells can give people a deeper understanding of the physical mechanism of cancer cell metastasis and spread.Studying the effect of anticancer drugs on the mechanical properties of cancer cells can make people more fully understand the mechanism of action of anticancer drugs,provide a certain reference for the development of new anticancer drugs,and have an important guiding role for the development of new drugs.