Comparing The Caspase-3 Activation Process Of Tumor Cells Induced By Anticancer Drugs

Tumor mainly stems from the inhibtion of cell apoptosis. And Caspases play an important role in apoptosis. Studing the Caspase-3 activation kinetics of tumor cells treated with anticancer drugs contributes to developing new anticancer drugs and illuminating the molecular mechanism of anticancer drugs. CD3 probe is a fluorescent probe consisting of CFP (Cyan fluorescent protein), DsRed (red fluorescent protein) and a linker with a recognition site of Caspase-3. In this dissertation, ACC-M, HepG2 and Hela cells stably expressing CD3 were treated with different anticancer drugs. With the self-made CE (capillary electrophoresis) system, the Caspase-3 activity was dectected.CD3-Hela cells were treated with the cell cycle-independent drug (cisplatin) and the cell cycle-dependent drug (camptothecin and etoposide), and then the Caspase-3 activity was detected with CE. The results indicated that the velocity of Caspase-3 activation induced by cisplatin had a positive correlation with its concentration. But the velocity of Caspase-3 activation induced by camptothecin and etoposide didn't show an apparent correlation with their concentration.Hela, HepG2 and ACC-M cells were treated with cisplatin, camptothecin and etoposide. And the Caspase-3 activity was detected with CE. The results indicated that different cells showed distinct sensitivity upon the same drug. ACC-M cells were more sensitive than other two cell types when induced by cisplatin. And HepG2 cells were most sensitive when treated by camptothecin and etoposide.For synergy experiments, Hela, HepG2 and ACC-M cells were treated with cisplatin, camptothecin and etoposide in combination with TNF (tumor necrosis factor). And the Caspase-3 activity was detected with CE. The results illustrated that combined treatment with anticancer drugs significiantly speed up the Caspase-3 activating process. And the enhancement was also various in different cell types. Above all, in this dissertation, using CE combined with CD3 probe, the Caspase-3 activation was detected when induced by anticancer drugs. And the Caspase-3 activation process was analysed. The result indicates that CE combined with CD3 probe can be used to study the anticancer mechanism of drugs and is also an effective method to screen anticancer drugs.