| Atherosclerosis(AS)is a potential pathological factor causing cardiovascular disease.Therefore,with the development of AS,it is becoming more and more globalized and younger.It is necessary to seek new drugs other than traditional treatment drugs and explore the reliability diagnostic targets of AS are extremely important.During the whole process of AS,mutations in specific genes and DNA will affect the development of AS.Studies have found that small micro RNAs(miRNAs)can regulate the expression of multiple target m RNAs at the post-transcriptional level,thereby regulating protein expression and regulateing a variety of diseases.Related miRNAs are involved in different stages of AS disease molecules.The traditional treatment of AS such as atorvastatin has adverse reactions such as liver toxicity and rhabdomyolysis,and is not suitable for long-term medication.As a new antioxidant emerging in 2007,hydrogen has significant physiological effects and good safety.It has various forms and has shown good therapeutic effects on a variety of disease models.A significant anti-AS effect was also demonstrated.Therefore,exploring the anti-AS mechanism of hydrogen will help us to further understand the mechanism of hydrogen action and seek possible targets for AS.In this paper,the concentration of hydrogen in the hydrogen-rich water storage solution prepared by the non-continuous hydrogen-passing device was monitored over time.The results show that the hydrogen concentration in the hydrogen-rich water storage solution will drop rapidly in a short period of time,and the hydrogen molecules are easy to escape.Compared the influence of the material of the hydrogen storage container and the amount of air in the hydrogen storage bottle on the hydrogen concentration,the results show that the glass container and the full bottle of liquid are more conducive to the preservation of hydrogen-rich wate.Therefore,glass bottle material and the state of full bottle of liquid were applied to build an in vitro hydrogen-rich cell culture device.The stability of the device was determined by measuring the hydrogen concentration and the cell activity detection when cells were cultured in the device.The results show that the device can stably deliver hydrogen without affecting cell growth activity,and can realize continuous and stable hydrogen-rich culture of cells in vitro.Furthermore,0,10,100,and 1000 ng/m L LPS were used to induce HUVEC,and various indicators such as cell activity,miRNA and adhesion molecule expression were detected to explore whether LPS could induce AS inflammatory cell model and the optimal concentration.The results showed that the concentration of 100ng/m L is the optimal induction concentration and HUVEC inflammation model could be successfully constructed.The effect of hydrogen on LPS-induced HUVEC was investigated compared with atorvastatin.The results show that hydrogen can promote the proliferation of HUVEC,up-regulate the expression of miRNA and inhibit the release of adhesion molecules,and the effect is comparable to that of atorvastatin.Therefore,hydrogen may regulate the expression of adhesion molecules by up-regulating the expression of related miRNAs.The mi R-17 and mi R-31,-126,-155,-221 are potential targets of hydrogen to promote HUVEC proliferation and inhibit the release of adhesion molecules. |
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